Passive Immunistion Of Pasteurella Multocida Infected Rabbits
By: Ali Ahmad
| Dr. Masood Rabbani, Asso.Prof., CDL.
Contributor(s): Dr. Khushi Muhammad, Associate Prof | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2003Subject(s): Department of MicrobiologyDDC classification: 0816,T Dissertation note: Haemorrhagic septicaemia (HS), an important bacterial disease of buffaloes and cattle, is caused by Pasteurella multocida. Improved management practices and regular vaccination programme have significantly contributed to lowering the incidence of the HS disease in our country. The outbreaks are mostly experienced in young animals, especially, calves (Sheikh et al., 1996). Present study was conducted to determine the contribution of passive immunization against HS in infected rabbits. In this study, 5 rabbits were vaccinated thrice at 21 days interval, using oil base haemorrhagic septicaemia vaccine (OBHSV). A high titre of anti-Pasteurella multocida-antibodies in blood serum was determined on 56th day post boosting using indirect haemagglutination (IHA) test. A virulent Pasteurella multocida with mean lethal dose (LD50) of 10-6.749 was used for challenging the rabbits. The hyperimmune serum (1 ml, 256 IHA units) injected intravenously to Pasteurella multocida challenged rabbit showed 100% protection as compared that of intramuscularly injected serum, which showed 66.66% protection. Similarly the antigen control group showed 0% protection. Moreover, it was found suitable to passively immunize the animals before exposure or simultaneously with the exposure of Pasteurella multocida. It is concluded that the hyperimmune serum can therapeutically be used to cure the Pasteurella multocida infected rabbits.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0816,T (Browse shelf) | Available | 0816,T |
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Haemorrhagic septicaemia (HS), an important bacterial disease of buffaloes and cattle, is caused by Pasteurella multocida. Improved management practices and regular vaccination programme have significantly contributed to lowering the incidence of the HS disease in our country. The outbreaks are mostly experienced in young animals, especially, calves (Sheikh et al., 1996). Present study was conducted to determine the contribution of passive immunization against HS in infected rabbits. In this study, 5 rabbits were vaccinated thrice at 21 days interval, using oil base haemorrhagic septicaemia vaccine (OBHSV). A high titre of anti-Pasteurella multocida-antibodies in blood serum was determined on 56th day post boosting using indirect haemagglutination (IHA) test. A virulent Pasteurella multocida with mean lethal dose (LD50) of 10-6.749 was used for challenging the rabbits. The hyperimmune serum (1 ml, 256 IHA units) injected intravenously to Pasteurella multocida challenged rabbit showed 100% protection as compared that of intramuscularly injected serum, which showed 66.66% protection. Similarly the antigen control group showed 0% protection. Moreover, it was found suitable to passively immunize the animals before exposure or simultaneously with the exposure of Pasteurella multocida. It is concluded that the hyperimmune serum can therapeutically be used to cure the Pasteurella multocida infected rabbits.
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