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Propagation Of Hydropericrdium Syndrome Virus In Laboratory Host Systems

By: Basharat Mahmood | Dr. Mansur-ud-Din Ahmad.
Contributor(s): Dr. Azhar | Dr. Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 2005Subject(s): Department of MicrobiologyDDC classification: 0880,T Dissertation note: Hydropericardium syndrome primarily affects the broilers between the age of 2-7 weeks. A vaccine prepared from infected liver extract treated with formaldehyde is being used to protect the broilers from the disease. The current study was carried out to propagate the hydropericardium syndrome virus in various laboratory host systems i.e. embryonated hen eggs, primary chicken embryo liver (CEL) cells, chicken embryo kidney (CKC) cells, chicken embryo (CEF) fibroblasts and BHK 21 cell line. The protocol for cultivation of primary chicken embryo liver cells, chicken embryo fibroblasts, chicken embryo kidney cells were standardized under local conditions. Liver samples were collected from HPS affected birds were processed and propagated in embryonated hen eggs, chicken embryo liver cells, chicken embryo kidney cells, chicken embryo fibroblasts and BHK-21 cell line. The comparative sensitivity to hydropericardium syndrome virus was studied. Five field samples were recovered out of seven after reproducing the disease in susceptible healthy broilers. These five liver samples were propagated in all laboratory host systems. It was recorded that hydropericardium syndrome virus could be propagated in chicken embryo liver cells and chicken embryo kidney cells. Hydropericardium syndrome virus could not be detected in AAF of embryonated hen eggs inoculated through allantoic, chicken embryo fibroblasts and BHK-21 cell line. Microtitration technique was used to determine the titer of the propagated virus. The serological techniques used to confirm the presence of HPS virus in cell culture supernatant and the allento- amniotic fluid were AGP test and serum neutralisation. Polyclonal antisera was raised using formalized liver homogenate vaccine and oil- based cell culture propagated vaccine. Polyclonal antiserum against HPS virus using oil-based cell culture propagated vaccine was found to Specific against liver homogenate collected from HPS affected birds.
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Hydropericardium syndrome primarily affects the broilers between the age of 2-7 weeks. A vaccine prepared from infected liver extract treated with formaldehyde is being used to protect the broilers from the disease. The current study was carried out to propagate the hydropericardium syndrome virus in various laboratory host systems i.e. embryonated hen eggs, primary chicken embryo liver (CEL) cells, chicken embryo kidney (CKC) cells, chicken embryo (CEF) fibroblasts and BHK 21 cell line. The protocol for cultivation of primary chicken embryo liver cells, chicken embryo fibroblasts, chicken embryo kidney cells were standardized under local conditions. Liver samples were collected from HPS affected birds were processed and propagated in embryonated hen eggs, chicken embryo liver cells, chicken embryo kidney cells, chicken embryo fibroblasts and BHK-21 cell line. The comparative sensitivity to hydropericardium syndrome virus was studied. Five field samples were recovered out of seven after reproducing the disease in susceptible healthy broilers. These five liver samples were propagated in all laboratory host systems. It was recorded that hydropericardium syndrome virus could be propagated in chicken embryo liver cells and chicken embryo kidney cells. Hydropericardium syndrome virus could not be detected in AAF of embryonated hen eggs inoculated through allantoic, chicken embryo fibroblasts and BHK-21 cell line. Microtitration technique was used to determine the titer of the propagated virus. The serological techniques used to confirm the presence of HPS virus in cell culture supernatant and the allento- amniotic fluid were AGP test and serum neutralisation. Polyclonal antisera was raised using formalized liver homogenate vaccine and oil- based cell culture propagated vaccine. Polyclonal antiserum against HPS virus using oil-based cell culture propagated vaccine was found to Specific against liver homogenate collected from HPS affected birds.

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