Development And Standardization Of Agar Gel Diffusion Test For Diagnosis Of Gigantocotyle Explanatum In Buffaloes
By: Shahzad Sharif
| Prof. Dr. H.A. Hashmi.
Contributor(s): Dr. Azhar Maqbool | Prof. Dr | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2005Subject(s): Department of ParasitologyDDC classification: 0919,T Dissertation note: Gigantocotyle explanaturn is one of the most prevalent paramphistome among the flukes which parasitize cattle and buffaloes. Diagnosis of parasitic diseases can be performed by coprological and immunological techniques. In the present study standardization of agar gel diffusion test (AGDT) was carried out. The soluble antigen of Gigantoco1'/e explaflatuni was prepared after homogenization of adult flukes in normi' saline and subsequent collection of supernatant after cenirifugation. 1-lyperimmunc senim against the antigen was rflise(l in rabbits. Agar gel plates were prepared and antigen was standardized against known positive serum. The faecal and blood samples were collected from 200 suspected buffaloes from two abattoirs of the Lahore city. AGDT was performed for serological identification of the samples and coprological examination for detection of ova. The recovery of flukes from the liver, bile duct and gall bladder of thc same animals was also carried out. 'l'he efficacy of serological and coprological examination for the diagnosis of G. explanatum was compared with that of direct recovery of parasite from liver of slaughtered animals.
Out of the 200 buffaloes 51 (25.5 percent) harhoured (7, explanatum in their livers, bile duct and gall bladder. The serological examination of the same animals showed that antibodies against (7. explanalum were detectable in 29 (56.85 percent) buffaloes by AGDT while the coprological examination of same animals revealed that ova of (i. explanatum were present in 23 (45.1 percent) out of the 51 animals. From these findings it can be inferred that efficacy of AGDT is superior to faecal examination and could become a useful technique for herd diagnosis of G. explandatum in buffaloes under field conditions.
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UVAS Library Thesis Section | Veterinary Science | 0919,T (Browse shelf) | Available | 0919,T |
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Gigantocotyle explanaturn is one of the most prevalent paramphistome among the flukes which parasitize cattle and buffaloes. Diagnosis of parasitic diseases can be performed by coprological and immunological techniques. In the present study standardization of agar gel diffusion test (AGDT) was carried out. The soluble antigen of Gigantoco1'/e explaflatuni was prepared after homogenization of adult flukes in normi' saline and subsequent collection of supernatant after cenirifugation. 1-lyperimmunc senim against the antigen was rflise(l in rabbits. Agar gel plates were prepared and antigen was standardized against known positive serum. The faecal and blood samples were collected from 200 suspected buffaloes from two abattoirs of the Lahore city. AGDT was performed for serological identification of the samples and coprological examination for detection of ova. The recovery of flukes from the liver, bile duct and gall bladder of thc same animals was also carried out. 'l'he efficacy of serological and coprological examination for the diagnosis of G. explanatum was compared with that of direct recovery of parasite from liver of slaughtered animals.
Out of the 200 buffaloes 51 (25.5 percent) harhoured (7, explanatum in their livers, bile duct and gall bladder. The serological examination of the same animals showed that antibodies against (7. explanalum were detectable in 29 (56.85 percent) buffaloes by AGDT while the coprological examination of same animals revealed that ova of (i. explanatum were present in 23 (45.1 percent) out of the 51 animals. From these findings it can be inferred that efficacy of AGDT is superior to faecal examination and could become a useful technique for herd diagnosis of G. explandatum in buffaloes under field conditions.
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