Isolation And Biological Characterization Of H7 Type Avian Influenza Virus
By: Syed Abid Hussain
| Dr. Muhammad Akram Muneer.
Contributor(s): Dr.Azhar | Dr.Masood Rabbani | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2006Subject(s): Department of MicrobiologyDDC classification: 0937,T Dissertation note: The present study was undertaken with the objectives to isolate and characterize avian influenza virus responsible for high morbidity and mortality in layers flocks in Karachi area of Pakistan. AIV H7 type was isolated from the morbid tissue samples by inoculating their tissue homogenate in the 9-1 1 day old developing chicken embryos. This isolate later declared as AIV H7 type, induced death of chicken embryos within 36 to 48 hours post inoculation. The type and subtype of the isolate was confirmed using known nionospecific antisera against various haemagglutinating viruses. Infectivity potential of AIV was determined by inoculating it in 9-11 day old embryos of layer, duck, desi-bird and pigeons. The AF from experimentally infected embryos, haernagglutinated the chicken red blood cells. However, the haemagglutination titer of virus in AF from eggs of various avian species was variable. For evaluating the survival/resistance of the virus against various chemical disinfectants, it was inoculated in embryonated chicken eggs following treatment of various dilutions disinfectants like Beloran, Zeptin and Formalin. These disinfectants were effective in inactivating the avian influenza virus at various concentration levels. For monitoring pathogenic characteristics, isolated virus was inoculated in the developing chicken embryos. The mean death time was evaluated as 42 hrs post inoculation. This investigation indicated that AIV-H7 type was widely circulating iii poultry flocks, and was causing high morbidity and mortality in the susceptible populations. The use of disinfectants like Beloran, Zeptin and F'orrnalin is suggested for inactivating the virus present in-and-around poultry farm premises.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0937,T (Browse shelf) | Available | 0937,T |
The present study was undertaken with the objectives to isolate and characterize avian influenza virus responsible for high morbidity and mortality in layers flocks in Karachi area of Pakistan. AIV H7 type was isolated from the morbid tissue samples by inoculating their tissue homogenate in the 9-1 1 day old developing chicken embryos. This isolate later declared as AIV H7 type, induced death of chicken embryos within 36 to 48 hours post inoculation. The type and subtype of the isolate was confirmed using known nionospecific antisera against various haemagglutinating viruses. Infectivity potential of AIV was determined by inoculating it in 9-11 day old embryos of layer, duck, desi-bird and pigeons. The AF from experimentally infected embryos, haernagglutinated the chicken red blood cells. However, the haemagglutination titer of virus in AF from eggs of various avian species was variable. For evaluating the survival/resistance of the virus against various chemical disinfectants, it was inoculated in embryonated chicken eggs following treatment of various dilutions disinfectants like Beloran, Zeptin and Formalin. These disinfectants were effective in inactivating the avian influenza virus at various concentration levels. For monitoring pathogenic characteristics, isolated virus was inoculated in the developing chicken embryos. The mean death time was evaluated as 42 hrs post inoculation. This investigation indicated that AIV-H7 type was widely circulating iii poultry flocks, and was causing high morbidity and mortality in the susceptible populations. The use of disinfectants like Beloran, Zeptin and F'orrnalin is suggested for inactivating the virus present in-and-around poultry farm premises.
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