Seropervalance Of Toxoplasma Gondii Infection In Indigenous Poultry And Their Dressers
By: Kashif Shahzad Hashmi | Prf.Dr. Azhar Maqbool.
Contributor(s): Dr. Kamran Ashraf (Assistant | Faculty of Veterinary Sciences.
Material type: BookPublisher: 2007Subject(s): Department of ParasitologyDDC classification: 0978,T Dissertation note: In present study a total of 100 serum samples from indigenous poultry and the dressers were analysed for determination of Toxoplasma specific anti-bodies. Under aseptic measures 1-2m1 of blood was withdrawn by veinpuncture and serum was separated. All the serum samples were analyzed for specific IgG anti Toxoplasma ant-bodies using Latex Agglutination Test (LAT). Positive reaction was visualized by the naked eyes or magnifying hand lens. Commercially available Toxoplasma Latex test kit was used to determine the serum antibodies. Results were interpretated, data obtained was analyzed statistically by calculating the percent sero-positivity of Toxoplasma gondii in indigenous poultry. Toxoplasma is one of the most common zoonosis worldwide.It is caused by toxoplasma gondii, an obligatory intracellular parasite that attacks warm blooded animals,birds including humanbiengs.Felines are the definite hosts where the parasite carries out its sexual cycle resulting in oocysts that are taken to the environment by the feces.Man birds and animals acquire the infection by ingesting oocysts that contaminate water,foodstuff, and pasture in that environment. In the present study the overall seroprevalance of toxoplasma gondii infection in indigenous poultry and dressers are 12% respectively by using Latex agglutination test. In indigenous poultry seroprevalance of toxoplasma gondii was 10% out of 100 hens examined, (3) were seropositive at screening dilution of 1:256 suggesting possible recent contact. (2) were seropositive at 1:128 which was due to acquired or evolving immunity, while (1) were seropositive at screening dilution at 1:16 indicating residual or non specific immunity. Toxoplasma gondii infection is increased with age because of constant exposure to contaminated environment and increased contact with cats. In dressers (butchers) seroprevalance was 12% out of 50.Two were seropositive at screening dilution of 1:256 suggesting possible recent contacting.Two were seropositive at 1:128 which was due to acquired or evolving immunity,whike two were seropositve at screening dilution at 1:16 indicating residual or non specific immunity. Due to increase prevalence of toxoplasmosis in humans,birds and animals daby day it is very necessary that public should be aware about the zoonotic importance of this disease and preventive measures to be adopted.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis | UVAS Library Thesis Section | Veterinary Science | 0978,T (Browse shelf) | Available | 0978,T |
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In present study a total of 100 serum samples from indigenous poultry and the dressers were analysed for determination of Toxoplasma specific anti-bodies. Under aseptic measures 1-2m1 of blood was withdrawn by veinpuncture and serum was separated. All the serum samples were analyzed for specific IgG anti Toxoplasma ant-bodies using Latex Agglutination Test (LAT). Positive reaction was visualized by the naked eyes or magnifying hand lens.
Commercially available Toxoplasma Latex test kit was used to determine the serum antibodies. Results were interpretated, data obtained was analyzed statistically by calculating the percent sero-positivity of Toxoplasma gondii in indigenous poultry.
Toxoplasma is one of the most common zoonosis worldwide.It is caused by toxoplasma gondii, an obligatory intracellular parasite that attacks warm blooded animals,birds including humanbiengs.Felines are the definite hosts where the parasite carries out its sexual cycle resulting in oocysts that are taken to the environment by the feces.Man birds and animals acquire the infection by ingesting oocysts that contaminate water,foodstuff, and pasture in that environment.
In the present study the overall seroprevalance of toxoplasma gondii infection in indigenous poultry and dressers are 12% respectively by using Latex agglutination test.
In indigenous poultry seroprevalance of toxoplasma gondii was 10% out of 100 hens examined, (3) were seropositive at screening dilution of 1:256 suggesting possible recent contact.
(2) were seropositive at 1:128 which was due to acquired or evolving immunity, while (1) were seropositive at screening dilution at 1:16 indicating residual or non specific immunity.
Toxoplasma gondii infection is increased with age because of constant exposure to contaminated environment and increased contact with cats.
In dressers (butchers) seroprevalance was 12% out of 50.Two were seropositive at screening dilution of 1:256 suggesting possible recent contacting.Two were seropositive at 1:128 which was due to acquired or evolving immunity,whike two were seropositve at screening dilution at 1:16 indicating residual or non specific immunity.
Due to increase prevalence of toxoplasmosis in humans,birds and animals daby day it is very necessary that public should be aware about the zoonotic importance of this disease and preventive measures to be adopted.
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