Disgnosis Of Theileriosis In Buffalo Through Blood Smear Examination And Pcr In District Lahore
By: Mukhtar Ahmad
| Dr.Jawaria Ali Khan.
Contributor(s): Prof.Dr.Muhammad Sarwar Khan | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2008Subject(s): Department of Clinical Medicine & SurgeryDDC classification: 0983,T Dissertation note: The present study was designed to determine diagnosis and infection percentage of
Theileriosis in buffalo in and around District Lahorë, and to design the primer for Theleria
parasite with Microscopic Examination. For this purpose blood samples were collected from
300 buffaloes randomly from 20 villages, during the month of May, June, July and August of
2007 in and around District Lahore. Screening was done by blood smears, stained by Giemsa’s
staining technique and later the blood sample from same animals was also processed by PCR.
The blood smear showed Theileria, piroplasms, including cocci, rod, and signet-ring, with diameter of 0.5-1.5 micrometer. The blood parameter i-e PCV, Hb concentration and TEC showed presence of microlytic hypochromic anemia in diseased animal. On the basis of microscopic examination overall 44.66% (134/300) prevalence was recorded. On the basis of PCR test prevalence of Theleria parasite 5 9.66% (179/300) was recorded show the presence of carrier animal in buffalo population in district Lahore. The result had shown high efficacy of PCR as compare to microscopic examination.
It is anticipated that present study was proved helpful in diagnosis of Theileria in infected as well as in carrier animals in District Lahore and will be beneficial for further study.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0983,T (Browse shelf) | Available | 0983,T |
The present study was designed to determine diagnosis and infection percentage of
Theileriosis in buffalo in and around District Lahorë, and to design the primer for Theleria
parasite with Microscopic Examination. For this purpose blood samples were collected from
300 buffaloes randomly from 20 villages, during the month of May, June, July and August of
2007 in and around District Lahore. Screening was done by blood smears, stained by Giemsa’s
staining technique and later the blood sample from same animals was also processed by PCR.
The blood smear showed Theileria, piroplasms, including cocci, rod, and signet-ring, with diameter of 0.5-1.5 micrometer. The blood parameter i-e PCV, Hb concentration and TEC showed presence of microlytic hypochromic anemia in diseased animal. On the basis of microscopic examination overall 44.66% (134/300) prevalence was recorded. On the basis of PCR test prevalence of Theleria parasite 5 9.66% (179/300) was recorded show the presence of carrier animal in buffalo population in district Lahore. The result had shown high efficacy of PCR as compare to microscopic examination.
It is anticipated that present study was proved helpful in diagnosis of Theileria in infected as well as in carrier animals in District Lahore and will be beneficial for further study.
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