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Diagnosis Of Surra In Equines By Indirection Fluorescent Anitobody

By: Malik Ahsan Nadeem | Dr.Asim Aslam.
Contributor(s): Dr.Kamran | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 2007Subject(s): Department of PathologyDDC classification: 0994,T Dissertation note: Trypanosomiasis (‘surra) is the most widely distributed arthropod- born protozoan disease affecting the equines. This study was conduàted to check the efficacy of indirect fluorescent antibody test (IFAT) for the diagnosis of Surra. For this purpose 200 blood samples were collected from horses and donkeys from different areas of Gujranwala district. Thin blood smears were prepared on clean glass slides and blood samples were centrifuged to separate the serum. Serum was transferred into the vacutainers and transported to laboratory. The serum was separated by centrifugation and stored at -70°C. 200 thin blood smear slides were fixed with methanol and subjected to Giemsa stain for further microscopic examination. Then the 200 thin blood smear slides were fixed with acetone for further processing in indirect fluorescent antibody test (IFAT). The prevalence rate of 2% and 6% by using thin blood smear and indirect fluorescent antibody test (IFAT) was obtained respectively. The results helped us to determine accuracy of indirect fluorescent antibody test (IFAT) for diagnosis of Surra.
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Trypanosomiasis (‘surra) is the most widely distributed arthropod- born protozoan disease affecting the equines. This study was conduàted to check the efficacy of indirect fluorescent antibody test (IFAT) for the diagnosis of Surra. For this purpose 200 blood samples were collected from horses and donkeys from different areas of Gujranwala district. Thin blood smears were prepared on clean glass slides and blood samples were centrifuged to separate the serum. Serum was transferred into the vacutainers and transported to laboratory. The serum was separated by centrifugation and stored at -70°C. 200 thin blood smear slides were fixed with methanol and subjected to Giemsa stain for further microscopic examination. Then the 200 thin blood smear slides were fixed with acetone for further processing in indirect fluorescent antibody test (IFAT). The prevalence rate of 2% and 6% by using thin blood smear and indirect fluorescent antibody test (IFAT) was obtained respectively. The results helped us to determine accuracy of indirect fluorescent antibody test (IFAT) for diagnosis of Surra.

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