Selection Of Thermostable Reconstituted Newcastle Disease Virus Progeny From Vaccinal Strain(La Sota)
By: Faiza Ghazanfar
| Prof.Dr.Mansur-ud-Din Ahmad.
Contributor(s): Prof.Dr.Mohammad Akram Munir | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2008Subject(s): Department of MicrobiologyDDC classification: 1040,T Dissertation note: Poultry sector is the second largest industry after textile in Pakistan. It is threatened by various diseases. Every year, Newcastle disease is causing enormous losses to this industry. A thermostable vaccine of Newcastle disease will be an answer to this problem.
A vaccine strain of Newcastle disease La Sota was divided into two groups BATCH-A and BATCH-B. BATCH-A was selected for sudden heat exposure at 45°C. But virus could not survive the direct exposure of this temperature. So BATCH-B was selected for gradual increasing heat shock treatment. The starting temperature was selected at 40°C for 20 minutes. Stable NDV virus at that temperature was subjected to further heat treatments at 42°C for 20, 25, 30, 35, 40, 45, 50, 55 and 60 minutes. Virus suspension was labeled as "BATCH-B 1".
BATCH-B 1 was divided into four groups for different heat treatments. They were labeled as Bl-A, B1-B, Bl-C and B1-D. B1-A was subjected to the temperature of 50°C for 20 minutes. B1-A titer was 2 (1:32). It showed drop in HA titer upto fourth well (1:16). B 1-B was placed at room temperature for three consecutive days with HA checking after every 6 hours. No drop in HA titer was observed. B 1-C was placed in a bright sunlight for 12 hours and HA titer was dropped down by one well (1 log2). B1-C HA titer was 2 (1 :32).It dropped down to 2 (1:16). Lastly, the parent virus suspension i.e. BATCH-B with HA titer of tenth well (1:1024) and virus suspension of "B 1 -D" with HA titer of fifth well (1:32) were placed in 56°C for 30 minutes, 60 minutes, 90 minutes and 120 minutes as described by King (2006) and Wambura et al (2006).
The virus suspension of "B 1 -D" survived the temperature but the titer dropped to third well (1:8) at 30 minutes. It was dropped further to 21(1:2) at 60 minutes and maintained its titer i.e. 21(1:2) at 90 minutes. The BATCH-B did not show any HA titer at 30 minutes and onwards. The E1D50 of B1-D was calculated as 10-7.4
The study will help to minimize the challenges at farm level. The vaccine can be transported in the rural areas as the thermostable strain which will be able to bear the harsh conditions of weather in Pakistan. Furthermore, its easy application will help the farmer to vaccinate his bird flock against this disease without any worries.
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UVAS Library Thesis Section | Veterinary Science | 1040,T (Browse shelf) | Available | 1040,T |
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Poultry sector is the second largest industry after textile in Pakistan. It is threatened by various diseases. Every year, Newcastle disease is causing enormous losses to this industry. A thermostable vaccine of Newcastle disease will be an answer to this problem.
A vaccine strain of Newcastle disease La Sota was divided into two groups BATCH-A and BATCH-B. BATCH-A was selected for sudden heat exposure at 45°C. But virus could not survive the direct exposure of this temperature. So BATCH-B was selected for gradual increasing heat shock treatment. The starting temperature was selected at 40°C for 20 minutes. Stable NDV virus at that temperature was subjected to further heat treatments at 42°C for 20, 25, 30, 35, 40, 45, 50, 55 and 60 minutes. Virus suspension was labeled as "BATCH-B 1".
BATCH-B 1 was divided into four groups for different heat treatments. They were labeled as Bl-A, B1-B, Bl-C and B1-D. B1-A was subjected to the temperature of 50°C for 20 minutes. B1-A titer was 2 (1:32). It showed drop in HA titer upto fourth well (1:16). B 1-B was placed at room temperature for three consecutive days with HA checking after every 6 hours. No drop in HA titer was observed. B 1-C was placed in a bright sunlight for 12 hours and HA titer was dropped down by one well (1 log2). B1-C HA titer was 2 (1 :32).It dropped down to 2 (1:16). Lastly, the parent virus suspension i.e. BATCH-B with HA titer of tenth well (1:1024) and virus suspension of "B 1 -D" with HA titer of fifth well (1:32) were placed in 56°C for 30 minutes, 60 minutes, 90 minutes and 120 minutes as described by King (2006) and Wambura et al (2006).
The virus suspension of "B 1 -D" survived the temperature but the titer dropped to third well (1:8) at 30 minutes. It was dropped further to 21(1:2) at 60 minutes and maintained its titer i.e. 21(1:2) at 90 minutes. The BATCH-B did not show any HA titer at 30 minutes and onwards. The E1D50 of B1-D was calculated as 10-7.4
The study will help to minimize the challenges at farm level. The vaccine can be transported in the rural areas as the thermostable strain which will be able to bear the harsh conditions of weather in Pakistan. Furthermore, its easy application will help the farmer to vaccinate his bird flock against this disease without any worries.
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