Effect On Lansoprazole On Antibacterial Activity Of Clarithromycin And Tetracyclin
By: Samreen Faisal
| Prof.Dr.Muhammad Ashraf.
Contributor(s): Dr.Aqeel Javeed | Dr.Sualeha Riffat.
Material type: 
BookPublisher: 2010Subject(s): Department of Pharmaoclogy & ToxicologyDDC classification: 1091,T Dissertation note: The study was designed to evaluate the effect of antibacterial drugs in the presence of lansoprazole (proton pump inhibitor) to determine the inhibition of bacterial efflux pumps. Resistance through efflux pump is one of the major mechanisms of resistance in bacteria. Bacillus ,subtilis, Salmonella typhi and Escherichia coli were isolated from clinical specimen and identified by using different microbiological techniques. Bacteria were inoculated separately in nutrient agar and their susceptibility for tetracycline and clarithromycin were checked by using Kirby baur disk diffusion method and the diameter of inhibitory zones (DIZ) was measured and analyzed by Unianova. The media were inoculated with three different concentrations of lansoprazole 1X, 2X and 3X separately and the same experiments were performed with each of the lansoprazole concentrations. The experiment was performed in five replicates. The diameter of inhibitory zone of tetracycline and clarithromycin in the presence of lansoprazole was measured in millimeters with the help of a scale and compared with the previously measured diameter of inhibitory zone of bacteria in absence of lansoprazole. Results of study showed that with increasing concentration of lansoprazole diameter of zone of inhibition of antibacterial drugs increases. The data suggest that the lansoprazole dose dependently increased the zone of inhibition of antibacterial drugs in the three test organisms used in study.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1091,T (Browse shelf) | Available | 1091,T |
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The study was designed to evaluate the effect of antibacterial drugs in the presence of lansoprazole (proton pump inhibitor) to determine the inhibition of bacterial efflux pumps. Resistance through efflux pump is one of the major mechanisms of resistance in bacteria. Bacillus ,subtilis, Salmonella typhi and Escherichia coli were isolated from clinical specimen and identified by using different microbiological techniques. Bacteria were inoculated separately in nutrient agar and their susceptibility for tetracycline and clarithromycin were checked by using Kirby baur disk diffusion method and the diameter of inhibitory zones (DIZ) was measured and analyzed by Unianova. The media were inoculated with three different concentrations of lansoprazole 1X, 2X and 3X separately and the same experiments were performed with each of the lansoprazole concentrations. The experiment was performed in five replicates. The diameter of inhibitory zone of tetracycline and clarithromycin in the presence of lansoprazole was measured in millimeters with the help of a scale and compared with the previously measured diameter of inhibitory zone of bacteria in absence of lansoprazole. Results of study showed that with increasing concentration of lansoprazole diameter of zone of inhibition of antibacterial drugs increases. The data suggest that the lansoprazole dose dependently increased the zone of inhibition of antibacterial drugs in the three test organisms used in study.
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