Effect Of Thawing At Ambient Temperature On The Qulty Of Frozen Buffalo Semen
By: Muhammad Nazir
| Dr. Imtiaz Hussain Khan.
Contributor(s): Dr. M. Yaqoob | Dr. Naeem Ullah Khan | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1985Subject(s): Department of TheriogenologyDDC classification: 1135,T Dissertation note: A total of 30 ejaculates, from ten normally producing Nili/Ravi buffalo bulls, with average fertility results, were obtained for the present study. Semen was extended in Lactose- Fructose-Yolk-Glycerol extender in a one step dilution. Semen packaged in 0.5 ml French straws was frozen and stored in liquid nitrogen for 24 hours before thawing.
Thawing of semen was carried out in a water bath at five ambient temperatures (20°, 25°, 30°,35° and 40°c) and three durations (15, 30 and 60 seconds). Thawing temperature of 37°C for 15 seconds was taken as control.
After each thawing, seminal quality was determined by each of the five testing methods i.e. % progressive motility of spermatozoa, percentage of intact acrosomes at 2 hours of incubation at 38 C, livability, Absolute index of livability, and the sephadex Gel-filteration test.
Analysis of data showed significant differences ( P<0.05) among all 16 thawing procedures on the basis of each evaluation methods used to determine the post-thaw seminal quality.
Analysis of variance revealed that the differences accounted for the temperature alone (P < 0.01) and not the thawing durations. (P>0.01).
Variations among bulls were lower than ejaculates and therefore, interactions of bulls with treatments were not expected (1> 0.05).
Multiple comparisons revealed highest values of seminal quality for all test methods after thawing at 40°C for 15 to 30 seconds.
As thawing of semen at lower temperatures resulted in inferior quality therefore, thawing at an ambient temperature below 35°C could not be recommended.
From the above results it may be concluded that to achieve maximal post-thaw seminal quality (and fertility) and to avoid sperm cell damage with cold shock, thawing of frozen buffalo bull semen be carried out at water bath temperature of 40°C for 15 and 30 seconds during Winter and Summer, respectively.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
|
UVAS Library Thesis Section | Veterinary Science | 1135,T (Browse shelf) | Available | 1135,T |
A total of 30 ejaculates, from ten normally producing Nili/Ravi buffalo bulls, with average fertility results, were obtained for the present study. Semen was extended in Lactose- Fructose-Yolk-Glycerol extender in a one step dilution. Semen packaged in 0.5 ml French straws was frozen and stored in liquid nitrogen for 24 hours before thawing.
Thawing of semen was carried out in a water bath at five ambient temperatures (20°, 25°, 30°,35° and 40°c) and three durations (15, 30 and 60 seconds). Thawing temperature of 37°C for 15 seconds was taken as control.
After each thawing, seminal quality was determined by each of the five testing methods i.e. % progressive motility of spermatozoa, percentage of intact acrosomes at 2 hours of incubation at 38 C, livability, Absolute index of livability, and the sephadex Gel-filteration test.
Analysis of data showed significant differences ( P<0.05) among all 16 thawing procedures on the basis of each evaluation methods used to determine the post-thaw seminal quality.
Analysis of variance revealed that the differences accounted for the temperature alone (P < 0.01) and not the thawing durations. (P>0.01).
Variations among bulls were lower than ejaculates and therefore, interactions of bulls with treatments were not expected (1> 0.05).
Multiple comparisons revealed highest values of seminal quality for all test methods after thawing at 40°C for 15 to 30 seconds.
As thawing of semen at lower temperatures resulted in inferior quality therefore, thawing at an ambient temperature below 35°C could not be recommended.
From the above results it may be concluded that to achieve maximal post-thaw seminal quality (and fertility) and to avoid sperm cell damage with cold shock, thawing of frozen buffalo bull semen be carried out at water bath temperature of 40°C for 15 and 30 seconds during Winter and Summer, respectively.
There are no comments for this item.