Effect Of Pregnant Mare'S Serum On Supervovulation In Rabbits
By: Robina Mumtaz
| Dr. Abrar Hussain Gilani.
Contributor(s): Dr. M.A | Dr. Muhammad Shoaib Akhtar | Faculty of Biosciences.
Material type: 
BookPublisher: 1981Subject(s): Department of PhysiologyDDC classification: 1142,T Dissertation note: An investigation was conducts on 42 rabbit does to study the effect of different dose levels of of Pregnant mere's Serum (PMS) on superovulation in rabbits. These animals were divided into seven groups, each group comprising of six animals. The first three groups were given PMS intramuscularly at the rate of 25, 50 end 75 lU per kg. body weight for three consecutive days. The fourth, fifth and sixth group received PMS at the ease rate but through subcutaneous route, and the dose administered subcutaneously was divided into two equal halves which were given t 8.00 A.M and 7.00 P.M. on the same day. The seventh group served as control end was injected only normal saline for three consecutive days. On the morning of the fourth day, each doe wee meted and was administered HCG at the rate of 50 IU per kg body weight. The does were slaughtered 24 hours after HCG administration. The ovaries were examined for corpora haemorrhagica, corpora lutea, graafian follicles and immature follicles. Oviducts and uterus was flushed with normal saline. The eggs so recovered were counted.
The maximum number of ova were recovered with 50 IU of PMS whether giver intramuscularly (12.0 + 2.30) or subcutaneously (18.16 + 1.47). The number of corpora haemorrhagica followed the same trend as that of egg recovery. The maximum count of corpora haemorrhagica was 35.67+4.64 when PMS was given subcutaneously which was approximately twice the number obtained intramuscularly. The number of corpora lutea and immature follicles were not significantly higher than that observed in control group. Maximum number (23.16 + 4.49) of greafian follicles was obtained with 50 IUof when administrated intramuscularly. The count of graafian follicles was follicles was almost the same with 50 and 75 IU of PMS when given subcutneous1y.
The effect of various doses of PMS upon ova recovery, corpora haemorrhagica and graafian follicles was found to be statistically significant whereas the differences of corpora lutea and immature follicles was statistically non-significant. A doss level of 50 IU of PMS administered subcutaneously may be recommended for supervulation in rabbit does.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1142,T (Browse shelf) | Available | 1142,T |
An investigation was conducts on 42 rabbit does to study the effect of different dose levels of of Pregnant mere's Serum (PMS) on superovulation in rabbits. These animals were divided into seven groups, each group comprising of six animals. The first three groups were given PMS intramuscularly at the rate of 25, 50 end 75 lU per kg. body weight for three consecutive days. The fourth, fifth and sixth group received PMS at the ease rate but through subcutaneous route, and the dose administered subcutaneously was divided into two equal halves which were given t 8.00 A.M and 7.00 P.M. on the same day. The seventh group served as control end was injected only normal saline for three consecutive days. On the morning of the fourth day, each doe wee meted and was administered HCG at the rate of 50 IU per kg body weight. The does were slaughtered 24 hours after HCG administration. The ovaries were examined for corpora haemorrhagica, corpora lutea, graafian follicles and immature follicles. Oviducts and uterus was flushed with normal saline. The eggs so recovered were counted.
The maximum number of ova were recovered with 50 IU of PMS whether giver intramuscularly (12.0 + 2.30) or subcutaneously (18.16 + 1.47). The number of corpora haemorrhagica followed the same trend as that of egg recovery. The maximum count of corpora haemorrhagica was 35.67+4.64 when PMS was given subcutaneously which was approximately twice the number obtained intramuscularly. The number of corpora lutea and immature follicles were not significantly higher than that observed in control group. Maximum number (23.16 + 4.49) of greafian follicles was obtained with 50 IUof when administrated intramuscularly. The count of graafian follicles was follicles was almost the same with 50 and 75 IU of PMS when given subcutneous1y.
The effect of various doses of PMS upon ova recovery, corpora haemorrhagica and graafian follicles was found to be statistically significant whereas the differences of corpora lutea and immature follicles was statistically non-significant. A doss level of 50 IU of PMS administered subcutaneously may be recommended for supervulation in rabbit does.
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