Rapid Detection Of Low Pathogenic Avain Influenza (H9) Viruses In Poultry Using Pt-Pcr And Its Comparison With Various Pathological Pictures
By: Kiren Aqil
| Dr. M. Younas Rana.
Contributor(s): Dr. Kamran | Dr. Mati Ur Rehman Khan.
Material type: 
BookPublisher: 2010Subject(s): Department of PathologyDDC classification: 1182,T Dissertation note: Total of 72 three-week old chickens were divided into two groups, group A and group B. Thirty Six chickens were placed in group B, infected with H9N2 influenza virus; while 36 chickens were placed in group A, control group. Organ and blood samples were collected daily from day 2 post infection to day 10 post infection. The birds were offered toxin free feed and water ad-libitum.
The infected birds did not show any pathogenic symptoms of disease. Gross pathological lesions were mainly hemorrhages in trachea and lungs. Slight to severe enteritis from day 6-9 post infection along with congestion of caecal tonsils was also seen.
Main histopathological lesions involved tracheal deciliation, infiltration of monocytes and neutrophils as well as vascular congestion in form of hemorrhagic area, cellular infiltration of inflammatory cells was seen in lungs. Necrotic foci, accumulation of inflammatory cells, sloughing of mucosa and infiltration of leucocytes in caecal tonsils was observed.
Hematological parameters i.e. TLC, DLC, Hemoglobin were measured and analyzed. It was interesting to find out that significant increase was noted in TLC of group B and hemoglobin concentration between chickens of Group A and B was found; however when DLC was conducted, there was significant increase in blood heterophils and decrease in monocytes.
RT-PCR was conducted to detect viral RNA in organ samples and it was detected as early as 48 hours post-infection in organs samples collected from infected chickens. Moreover, we have detected viral RNA in organ samples until day 10 post-infection.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1182,T (Browse shelf) | Available | 1182,T |
Total of 72 three-week old chickens were divided into two groups, group A and group B. Thirty Six chickens were placed in group B, infected with H9N2 influenza virus; while 36 chickens were placed in group A, control group. Organ and blood samples were collected daily from day 2 post infection to day 10 post infection. The birds were offered toxin free feed and water ad-libitum.
The infected birds did not show any pathogenic symptoms of disease. Gross pathological lesions were mainly hemorrhages in trachea and lungs. Slight to severe enteritis from day 6-9 post infection along with congestion of caecal tonsils was also seen.
Main histopathological lesions involved tracheal deciliation, infiltration of monocytes and neutrophils as well as vascular congestion in form of hemorrhagic area, cellular infiltration of inflammatory cells was seen in lungs. Necrotic foci, accumulation of inflammatory cells, sloughing of mucosa and infiltration of leucocytes in caecal tonsils was observed.
Hematological parameters i.e. TLC, DLC, Hemoglobin were measured and analyzed. It was interesting to find out that significant increase was noted in TLC of group B and hemoglobin concentration between chickens of Group A and B was found; however when DLC was conducted, there was significant increase in blood heterophils and decrease in monocytes.
RT-PCR was conducted to detect viral RNA in organ samples and it was detected as early as 48 hours post-infection in organs samples collected from infected chickens. Moreover, we have detected viral RNA in organ samples until day 10 post-infection.
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