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Production Of Methane Gas From Effluent Of A.B Murie By Methanobacterium Ruminantium

By: Huma Shabbir | Dr.Abu Saeed Hashmi.
Contributor(s): Miss Saeeda | Miss Shagufta Saeed.
Material type: materialTypeLabelBookPublisher: 2011Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1285,T Dissertation note: There is extreme shortage of energy in our country. Homes, private sectors and industries are suffering a lot due to this shortage. There are different sources of energy like fossil fuels which include coal, petroleum and natural gas etc. Apart from it there are number of renewable sources but fact is that all these sources are getting diminished because of excessive use due to growing population of the world. In order to increase energy in our country it is imperative to utilize the industrial waste. At present a lot of industrial effluent is being wasted which can be used for the production of fuel in the form of methane gas. The production of methane was carried out by anaerobic fermentation by Methanobacterium ruminantium culturing on Effluent of A.B Murie. Before its production the proximate analysis of effluent was carried out to know its inherent nutritional potential. The fermented organism Methanobacterium ruminantium was isolated from feces of buffalo bull. The optimizing conditions of growth medium such as temperature, pH, ionic concentration of growth medium (Ca+2, Mg+2) and Carbon-Nitrogen ratio in the medium, for maximum methane production was determined on micro scale. Detection and estimation of methane was carried out by burning and by measuring pressure on pressure gauge. The optimum conditions thus determined on micro scale was applied on higher scale in a 3 liter flask. The pressure of methane gas obtained was 1.3 psi. It is therefore concluded that effluent of A.B Murie contain sufficient potential of methane production. The methane thus produced can be used as a fuel and light source for their own use.
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Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 1285,T (Browse shelf) Available 1285,T
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There is extreme shortage of energy in our country. Homes, private sectors and industries are suffering a lot due to this shortage. There are different sources of energy like fossil fuels which include coal, petroleum and natural gas etc. Apart from it there are number of renewable sources but fact is that all these sources are getting diminished because of excessive use due to growing population of the world. In order to increase energy in our country it is imperative to utilize the industrial waste. At present a lot of industrial effluent is being wasted which can be used for the production of fuel in the form of methane gas.

The production of methane was carried out by anaerobic fermentation by Methanobacterium ruminantium culturing on Effluent of A.B Murie. Before its production the proximate analysis of effluent was carried out to know its inherent nutritional potential. The fermented organism Methanobacterium ruminantium was isolated from feces of buffalo bull. The optimizing conditions of growth medium such as temperature, pH, ionic concentration of growth medium (Ca+2, Mg+2) and Carbon-Nitrogen ratio in the medium, for maximum methane production was determined on micro scale.

Detection and estimation of methane was carried out by burning and by measuring pressure on pressure gauge. The optimum conditions thus determined on micro scale was applied on higher scale in a 3 liter flask. The pressure of methane gas obtained was 1.3 psi.
It is therefore concluded that effluent of A.B Murie contain sufficient potential of methane production. The methane thus produced can be used as a fuel and light source for their own use.

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