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Comparative Efficiency Of Routine Identification Methods With Molecular Technique (Pcr) For Detection Of Caecal Eimeria Species in Broilers

By: Muhammad Yasir | Prof. Dr. Kamran Ashraf.
Contributor(s): Dr. Aftab | Prof. Dr. Azhar Maqbool.
Material type: materialTypeLabelBookPublisher: 2011Subject(s): Department of ParasitologyDDC classification: 1274,T Dissertation note: Caecl coccidiosis is caused by Eimeria tenella and E. necatrix and poultry industry is facing huge economic losses due to this infection world wide. Eimeria tenella is the most common cause of caecal coccidiosis but E. necatrix is also isolated rarely. In the present study, 400 caecal samples suspected for coccidiosis were collected from two districts (Lahore and Gujranwala). Ten samples from twenty farms of each district were collected and examined for coccidiosis and species identication. Prevalence of coccidiosis was found 68%. It was more prevalent in district Gujranwala (71.5%) as compared to Lahore (64.5%). Eimeria species were identified by conventional (Direct Microscopy, Sedimentation technique, Floatation Technique and Sporulation) and molecular technique (PCR). Polymerase chain reaction was found the most sensitive ands accurate technique for species identification as compared to all conventional techniques. The prevalence percentage of E.tenella and E,necatrix was found 68% and 12.5% respectively. E.tenella was more prevalent in district Gujranwala (71.5%) as compared to district Lahore (64.5%). E.necatrix was also more prevalent in district Gujranwala (13.5%) than district Lahore (11.5%). The difference in percent prevalence of coccidiosis and species of Eimeria may be due to difference in farm management, farming type, biosecurity measures.
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Caecl coccidiosis is caused by Eimeria tenella and E. necatrix and poultry industry is facing huge economic losses due to this infection world wide. Eimeria tenella is the most common cause of caecal coccidiosis but E. necatrix is also isolated rarely. In the present study, 400 caecal samples suspected for coccidiosis were collected from two districts (Lahore and Gujranwala). Ten samples from twenty farms of each district were collected and examined for coccidiosis and species identication. Prevalence of coccidiosis was found 68%. It was more prevalent in district Gujranwala (71.5%) as compared to Lahore (64.5%). Eimeria species were identified by conventional (Direct Microscopy, Sedimentation technique, Floatation Technique and Sporulation) and molecular technique (PCR). Polymerase chain reaction was found the most sensitive ands accurate technique for species identification as compared to all conventional techniques. The prevalence percentage of E.tenella and E,necatrix was found 68% and 12.5% respectively. E.tenella was more prevalent in district Gujranwala (71.5%) as compared to district Lahore (64.5%). E.necatrix was also more prevalent in district Gujranwala (13.5%) than district Lahore (11.5%). The difference in percent prevalence of coccidiosis and species of Eimeria may be due to difference in farm management, farming type, biosecurity measures.

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