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Surveillance Of Tuberculosis In Buffaloes, Cattle And Derectton Of Mycobacterium Bovis And Mycobacterium Tuberculosis in Food of Animal Origin

By: Muhammad Yasin Tipu | Prof. Dr. Zafar Iqbal Chaudhry.
Contributor(s): Dr. Muhammad Younus | Prof. Dr.
Material type: materialTypeLabelBookPublisher: 2010Subject(s): Department of Pathology | Phd. thesisDDC classification: 1321,T Dissertation note: The main objectives of this study were: to survey the prevalence of TB infection in livestock and their products in Pakistan; to standardize PCR based techniques for the detection of TB in buffaloes, cattle and animal products (milk and meat) as presently no such system has been developed for the detection of TB in animals and their products in Pakistan; to evaluate improved tests for the differentiation of Mycobacterium complex isolates in cattle, buffaloes and animal food products and to compare modern and conventional methods for rapid diagnosis of the Mycobacterial spp. The study was performed in different experiments to have surveillance of tuberculosis in Buffaloes and Cattle; and to detect the presence of different Mycobacteria in animal food products. One thousand animals from different areas of Lahore District were screened with the tuberculin test. The milk and blood of tuberculin tested animals were further studied for the presence of Mycobacterial spp. by conventional methods as well as Polymerase Chain Reaction (PCR). In other experiments one hundred market milk samples and ten thousand five hundred tissue samples from twenty-one hundred carcasses at Lahore slaughter house were screened with conventional microbiological tests and multiplex PCR for differentiation of Mycobacterium species. The results indicated that PCR had more sensitivity and required less time to detect and differentiate different Mycobacterial species as compared to conventional methods. It was also noted that M. bovis were found in milk and blood of milking animals as well as tissue sample collected from Lahore slaughter house. On the basis of findings, regular monitoring of the milking animals, animals to be slaughtered, and workers handling these animals is suggested. It is also recommended to review the current slaughter act to prevent the slaughtering of TB affected animals.
List(s) this item appears in: Phd. Theses
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Thesis Thesis UVAS Library
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Veterinary Science 1321,T (Browse shelf) Available 1321,T
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The main objectives of this study were: to survey the prevalence of TB infection in livestock and their products in Pakistan; to standardize PCR based techniques for the detection of TB in buffaloes, cattle and animal products (milk and meat) as presently no such system has been developed for the detection of TB in animals and their products in Pakistan; to evaluate improved tests for the differentiation of Mycobacterium complex isolates in cattle, buffaloes and animal food products and to compare modern and conventional methods for rapid diagnosis of the Mycobacterial spp. The study was performed in different experiments to have surveillance of tuberculosis in Buffaloes and Cattle; and to detect the presence of different Mycobacteria in animal food products. One thousand animals from different areas of Lahore District were screened with the tuberculin test. The milk and blood of tuberculin tested animals were further studied for the presence of Mycobacterial spp. by conventional methods as well as Polymerase Chain Reaction (PCR). In other experiments one hundred market milk samples and ten thousand five hundred tissue samples from twenty-one hundred carcasses at Lahore slaughter house were screened with conventional microbiological tests and multiplex PCR for differentiation of Mycobacterium species. The results indicated that PCR had more sensitivity and required less time to detect and differentiate different Mycobacterial species as compared to conventional methods. It was also noted that M. bovis were found in milk and blood of milking animals as well as tissue sample collected from Lahore slaughter house. On the basis of findings, regular monitoring of the milking animals, animals to be slaughtered, and workers handling these animals is suggested. It is also recommended to review the current slaughter act to prevent the slaughtering of TB affected animals.

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