Isolation And Antimicrobial Sensitivity Pattern Of Bacteria Associated With Diarrhea Among Children
By: Nida Shaukat
| Dr. Imran Najeeb.
Contributor(s): Dr,Afab Ahmad Anjum | Dr.Atif Hanif.
Material type: 
BookPublisher: 2011Subject(s): Department of MicrobiologyDDC classification: 1353,T Dissertation note: Diarrheal disease is one of the leading cause of morbidity and mortality among
children in underdeveloped countries. In the present study 140 stool samples, were
collected from cases of children diarrhea and 120 (85.71 %) samples showed bacterial
growth. From these culture positive samples, bacterial pathogens were isolated and
identified as per standard protocols described in Bergey's Manual of Determinative
Bacteriology. Out of 120 stool samples, 163 bacterial isolates were obtained as
Escherichia coli 113 (69.3%), Salmonella enterica 42 (25.8%) and Shigella species 8
(4.9%). From a total of 113 E. coli isolates, 48 (42.5%) were identified as invasive E. coli
and 65 (57.5%) were non-invasive on the basis of binding with the Congo Red dye of the
Medium. Age-wise prevalence of isolates was also analysed as bacterial pathogens were
found more in age group 1 month to 4 years (95.7%), followed by the least isolated from
age group 5 to 8 years (1.84%) and age group 9 to 12 years (2.4%). Antimicrobial
sensitivity profile, was studied by standard Disk diffusion method (Kirby Bauer) for
commonly used antibiotics, showed that all bacterial isolates were more sensitive to
antibiotics amikacin, norfloxacin, ciprofloxacin, imipenem, tazocin and less sensitive to
cephradine, doxycycline, tetracycline and augmentin. The present study findings showed
that although there are a number of causative agents like viruses, bacteria and parasites of
diarrheal disease, bacteria still remain one of the major cause with E. coli, Salmonella
and Shigella being more important bacterial pathogens among pediatric diarrheal patients
in the selected study of four different public sector hospitals in Lahore District.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
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UVAS Library Thesis Section | Veterinary Science | 1353,T (Browse shelf) | Available | 1353,T |
Diarrheal disease is one of the leading cause of morbidity and mortality among
children in underdeveloped countries. In the present study 140 stool samples, were
collected from cases of children diarrhea and 120 (85.71 %) samples showed bacterial
growth. From these culture positive samples, bacterial pathogens were isolated and
identified as per standard protocols described in Bergey's Manual of Determinative
Bacteriology. Out of 120 stool samples, 163 bacterial isolates were obtained as
Escherichia coli 113 (69.3%), Salmonella enterica 42 (25.8%) and Shigella species 8
(4.9%). From a total of 113 E. coli isolates, 48 (42.5%) were identified as invasive E. coli
and 65 (57.5%) were non-invasive on the basis of binding with the Congo Red dye of the
Medium. Age-wise prevalence of isolates was also analysed as bacterial pathogens were
found more in age group 1 month to 4 years (95.7%), followed by the least isolated from
age group 5 to 8 years (1.84%) and age group 9 to 12 years (2.4%). Antimicrobial
sensitivity profile, was studied by standard Disk diffusion method (Kirby Bauer) for
commonly used antibiotics, showed that all bacterial isolates were more sensitive to
antibiotics amikacin, norfloxacin, ciprofloxacin, imipenem, tazocin and less sensitive to
cephradine, doxycycline, tetracycline and augmentin. The present study findings showed
that although there are a number of causative agents like viruses, bacteria and parasites of
diarrheal disease, bacteria still remain one of the major cause with E. coli, Salmonella
and Shigella being more important bacterial pathogens among pediatric diarrheal patients
in the selected study of four different public sector hospitals in Lahore District.
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