To Study The Extraction, Purification And Characterization Of Papain Form Carica Papaya
By: Hafiz Anis-Ur-Rehman Tariq
| Miss Faiza Masood.
Contributor(s): Dr. Abu Saeed Hadhmi | Mir.
Material type: 
BookPublisher: 2011Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1362,T Dissertation note: In this piece of research work, the Extraction, Purification and Characterization of Papain (a proteolytic enzyme) from Papaya fruit (Carica Papaya L. of Caricaceae family) were carried out. For this purpose, the Peelings, Flesh and Seeds of the Papaya fruit were used. Extraction of Papain was done using 0.1M alkaline Phosphate buffer. Purification of Papain was carried out by Ammonium Sulphate precipitation and dialysis followed by Gel filtration by Sephadex G-75. Then Characterization of Papain such as protein estimation, determination of proteolytic activity (International Unit) of enzyme and SDS-PAGE analysis were performed to determine the molecular weight. Finally, the yield and proteolytic activity of the Papain obtained from the Peelings, Flesh and Seeds of the Papaya fruit was measured and compared with the commercial product available in the market.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1362,T (Browse shelf) | Available | 1362,T |
In this piece of research work, the Extraction, Purification and Characterization of Papain (a proteolytic enzyme) from Papaya fruit (Carica Papaya L. of Caricaceae family) were carried out. For this purpose, the Peelings, Flesh and Seeds of the Papaya fruit were used. Extraction of Papain was done using 0.1M alkaline Phosphate buffer. Purification of Papain was carried out by Ammonium Sulphate precipitation and dialysis followed by Gel filtration by Sephadex G-75. Then Characterization of Papain such as protein estimation, determination of proteolytic activity (International Unit) of enzyme and SDS-PAGE analysis were performed to determine the molecular weight. Finally, the yield and proteolytic activity of the Papain obtained from the Peelings, Flesh and Seeds of the Papaya fruit was measured and compared with the commercial product available in the market.
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