Detection Of Bovine Viral Diarrhea Virus Prevalent In Dairy Herds Of Punjab, Pakistan
By: Humayun Gohar
| Dr. Masood rabbani.
Contributor(s): Dr. Arfan ahmad | Factuly of veterinary science.
Material type: 
BookPublisher: 2011Subject(s): Department of MicrobiologyDDC classification: 1386,T Dissertation note: Mucosal disease virus (MDV)is a positive senseRNA virus having genome of
approximately 12.3 kb in length and is one of the most insidious and ubiquitous virus of
bovines throughout the world.The presence of persistently infected animals having no clinical
signs, need rapid screening whether they carry infectious agent or not. Now-a-days many
serological procedures and virus isolation techniques are used for diagnosis of bovine viral
diarrhea virus (BVDV) infected animals.BVDV is very difficult, laborious and time consuming
to isolate in cell culture or in laboratory animal. But it can be substituted by antigen capture
ELISA method which is very specific as well as sensitive and easy to perform. Therefore, the
detection of mucosal disease virus (MDV) by Antigen Capture ELISAhas become a sound
alternative to routine tests. Antigen Capture ELISAidentifies BVDV infected animals by
detecting virus in serum and blood samples, etc. The study was organized to detect BVDV in
dairy animals at selected areas Punjab and to evaluate comparative prevalence of BVDV in
cattle and buffaloes.One hundred and eighty four bovine blood samples were collected from
both public and private livestock farms. Blood samples were collected from Military Farms,
Lahore, BRI (Buffalo Research Institute, Pattoki), LPRI (Livestock Production Research
Institute, Bahadarnagar, Okara), Government Livestock Farm, Rakh-Ghulamman, Kalurkot and
from private sector,Rizi dairiesChunia, Supreme Farm Multan Road, Lahore and Livestock and
Dairy Products Farm lumber, Lahore. The collected serum samples were screened for detecting
virus through indirect antigen capture ELISA.lt showed that 16.85% cattle and 6.31 % buffalo
were found positive for BVDV.lt was also found that prevalence of BVDV infection was lower
at private farms as compared to Government farms. Lowest prevalence (0%) was found in
Friesian cow, whereas, highest prevalence (50%) was found in Sahiwal cattle. The cross bred
animals were also infected by BVDV infection. Overall prevalence of BVDV infection in
buffalo was lower than cattle population.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
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UVAS Library Thesis Section | Veterinary Science | 1386,T (Browse shelf) | Available | 1386,T |
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Mucosal disease virus (MDV)is a positive senseRNA virus having genome of
approximately 12.3 kb in length and is one of the most insidious and ubiquitous virus of
bovines throughout the world.The presence of persistently infected animals having no clinical
signs, need rapid screening whether they carry infectious agent or not. Now-a-days many
serological procedures and virus isolation techniques are used for diagnosis of bovine viral
diarrhea virus (BVDV) infected animals.BVDV is very difficult, laborious and time consuming
to isolate in cell culture or in laboratory animal. But it can be substituted by antigen capture
ELISA method which is very specific as well as sensitive and easy to perform. Therefore, the
detection of mucosal disease virus (MDV) by Antigen Capture ELISAhas become a sound
alternative to routine tests. Antigen Capture ELISAidentifies BVDV infected animals by
detecting virus in serum and blood samples, etc. The study was organized to detect BVDV in
dairy animals at selected areas Punjab and to evaluate comparative prevalence of BVDV in
cattle and buffaloes.One hundred and eighty four bovine blood samples were collected from
both public and private livestock farms. Blood samples were collected from Military Farms,
Lahore, BRI (Buffalo Research Institute, Pattoki), LPRI (Livestock Production Research
Institute, Bahadarnagar, Okara), Government Livestock Farm, Rakh-Ghulamman, Kalurkot and
from private sector,Rizi dairiesChunia, Supreme Farm Multan Road, Lahore and Livestock and
Dairy Products Farm lumber, Lahore. The collected serum samples were screened for detecting
virus through indirect antigen capture ELISA.lt showed that 16.85% cattle and 6.31 % buffalo
were found positive for BVDV.lt was also found that prevalence of BVDV infection was lower
at private farms as compared to Government farms. Lowest prevalence (0%) was found in
Friesian cow, whereas, highest prevalence (50%) was found in Sahiwal cattle. The cross bred
animals were also infected by BVDV infection. Overall prevalence of BVDV infection in
buffalo was lower than cattle population.
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