Normal view MARC view ISBD view

Identification Of Single Nucleotide Polymorphisms In Olri Gene And Its Association With Milk Composition In Cattle Breeds in Pakistan

By: Nusrat Majeed | Prof.Dr.Masroor Elahi Babar.
Contributor(s): Dr. Ali Raza Awan | Prof. Dr. Abu.
Material type: materialTypeLabelBookPublisher: 2011Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1396,T Dissertation note: Pakistan being agriculture based country has a great potential in livestock sector. it plays an important role in the economy of the country. Milk is a balanced diet because it contains all the essential nutrients like carbohydrates; fats, proteins, vitamins, minerals and enzymes required for health. In these milk nutrients fat is second most important component of milk. Primary component of milk fat is triglycerides (triacylglycerols or TAG) a typical storage form of lipids. OLRI is the major protein that binds, internalizes and degrades oxidized low density lipoprotein. Ol.Rl as a protein important for oLDL metabol ism may contribute to these effects. Oxidized fat inhibits the expression of lipoprotein lipase and fatty acid transporter genes that causes a reduced uptake of fatty acids into mammary glands. As a result the concentration of triacylglycerols in milk is reduced. OLRl as a protein important for oLOL metabolism may contribute to these effects. OLR J identified as a functional and positional candidate gene product for milk fat percentage and milk fat yield. Polymorphism in Ol.Rl gene increases the milk fat percentage. If polymorphism in Ol.Rl gene is found in Pakistani Sahiwal and Ohanni cattle breeds these were help to screen the animals at younger age and also be used to characterize the different cattle breeds as a milk fat production marker. Unrelated animals were selected for this study. Blood samples were collected from different Go\'1. livestock farms/experimental stations. DNA was extracted by organic method. Primers were designed by using Primer3 software. After DNA amplification by polymerase chain reaction. peR product was sequenced. Sequencing results of the full length OLR I gene were analyzed by alignment of sequences with the help of Blast2sequence software. Novel S Ps were identified. Total of twenty one SNPs were identified in the samples of all breeds that were confirmed at population level by sequencing of each sample. Then total twenty one SNPs were found in all breeds. Most of SNPs were found in intronic regions. In Sahiwal only 5 SNPs are exonic and one of them is common in both Sahiwal and Red Sindhi breeds. Out of total five exonic SNPs, only two were found synonymous and rest of the three exonic SNPs were found to be non synonymous. while all other SNPs are intronic in nature. An intronic SNP is also common between Sahiwal and Dhanni breed. In Red Sindhi a SNP was observed at 3'UTR. Results were analyzed by. using the statistical software POPgene32. The mean value of expected heterozygosity that is 0.3595 is greater than the mean of observed heterozygosity that is 0.0300 that shows low variabil ity in breeds. The mean value for Shannon's Information index (1*) is 0.5421. The aim of research is to identify gene that underlie the genetic variation in bovine milk. fat composition.
Tags from this library: No tags from this library for this title. Add tag(s)
Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 1396,T (Browse shelf) Available 1396,T
Total holds: 0

Pakistan being agriculture based country has a great potential in livestock sector. it plays
an important role in the economy of the country. Milk is a balanced diet because it
contains all the essential nutrients like carbohydrates; fats, proteins, vitamins, minerals
and enzymes required for health. In these milk nutrients fat is second most important
component of milk. Primary component of milk fat is triglycerides (triacylglycerols or
TAG) a typical storage form of lipids. OLRI is the major protein that binds, internalizes
and degrades oxidized low density lipoprotein. Ol.Rl as a protein important for oLDL
metabol ism may contribute to these effects. Oxidized fat inhibits the expression of
lipoprotein lipase and fatty acid transporter genes that causes a reduced uptake of fatty
acids into mammary glands. As a result the concentration of triacylglycerols in milk is
reduced. OLRl as a protein important for oLOL metabolism may contribute to these
effects. OLR J identified as a functional and positional candidate gene product for milk fat
percentage and milk fat yield. Polymorphism in Ol.Rl gene increases the milk fat
percentage. If polymorphism in Ol.Rl gene is found in Pakistani Sahiwal and Ohanni
cattle breeds these were help to screen the animals at younger age and also be used to
characterize the different cattle breeds as a milk fat production marker. Unrelated animals
were selected for this study. Blood samples were collected from different Go\'1. livestock
farms/experimental stations. DNA was extracted by organic method. Primers were
designed by using Primer3 software. After DNA amplification by polymerase chain
reaction. peR product was sequenced. Sequencing results of the full length OLR I gene
were analyzed by alignment of sequences with the help of Blast2sequence software.
Novel S Ps were identified. Total of twenty one SNPs were identified in the samples of
all breeds that were confirmed at population level by sequencing of each sample. Then
total twenty one SNPs were found in all breeds. Most of SNPs were found in intronic
regions. In Sahiwal only 5 SNPs are exonic and one of them is common in both Sahiwal
and Red Sindhi breeds. Out of total five exonic SNPs, only two were found synonymous
and rest of the three exonic SNPs were found to be non synonymous. while all other
SNPs are intronic in nature. An intronic SNP is also common between Sahiwal and
Dhanni breed. In Red Sindhi a SNP was observed at 3'UTR. Results were analyzed by.
using the statistical software POPgene32. The mean value of expected heterozygosity that
is 0.3595 is greater than the mean of observed heterozygosity that is 0.0300 that shows
low variabil ity in breeds. The mean value for Shannon's Information index (1*) is 0.5421.
The aim of research is to identify gene that underlie the genetic variation in bovine milk.
fat composition.

There are no comments for this item.

Log in to your account to post a comment.


Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.