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Evaluation Of Adulticidal And Larvicidal Efficacy Of Zadirachta Indica (Neem) And Lantana Camara (Punch Phul) Extracts

By: Asemah Anwar | Prof. Dr. Azhar Maqbool.
Contributor(s): Dr. Aneela | Dr. Nisar Ahmad.
Material type: materialTypeLabelBookPublisher: 2012Subject(s): Department of ParasitologyDDC classification: 1475,T Dissertation note: Pakistan is one of sub-tropical countries and its climatic conditions favor parasitic diseases along with disease vectors including mosquitoes. Mosquitoes of genus Aedes are causing Dengue fever. It has become one of infectious vector born disease of world. Annually it is striking around 100 million people with dengue fever and about 5 Lac people with Dengue Hemorrhagic Fever (DHF), resulting in 5 % deaths per year. Current study was conducted to evaluate efficacies of plant extracts against dengue fever mosquitoes of Aedes genus. Study target was to search some safer alternates than that of chemical pesticides. Also exhibiting characteristics like less toxic to environment, do not induce resistance in mosquitoes, do not harm non-targeted organisms, would not be toxic to human beings and will have biodegradation ability. Study has evaluated indigenous plants extracts as potent larvicidal and adulticidal materials. Mosquitoes were collected from different areas of Lahore and they were identified upto genus level only Aedes genus was identified upto species level, then Aedes aegypti was reared in laboratory to have F 1 generation, to have mosquitoes and larvae for testing the activity of plant extracts. Plant materials were collected from different areas of Lahore. Methanolic extracts of seeds and leaves of Azadirachta indica and lantana camara respectively, were extracted accordingly. Stock solutions were prepared from these extracts and then out of this serial dilutions were made. Experiment was performed in four groups A, B, C & D for both adults and larvae. Each group was representing total of 25 larvae/adult out of which one was kept untreated as negative control, one with deltamethrin as positive control while two were provided with test concentrations. LC50 and LC90 was determined by Probit analysis, using SPSS version 13.0 SPSS inc. 2004. lethal concentrations of A. indica mosquitocidal assay were LC50=30.44 mg/l and LC90=62.36 mg/l after 24 hrs and LC50=-9.87mg/l and LC90=59.102 mg/l after 48 hrs at ?= 0.05. X 2 value was significant for 24 h while for 48 h it was not significant. Maximum mortality observed after 24 and 48 hrs is 99% at 70 ppm. DMR test shows after 24 hrs., all treatment means are significantlt different from each other, from control and from mean of insecticide's mortality, control is significantly lower while insecticide treated group has significantly higher than rest. after 48 hrs. treatments were significantly different from each other while treatment mortality at 70 PPM is significantly higher than insecticide treated group and rest. Lethal concentrations for L. camara adulticidal assay were LC50=60.40 mg/l and LC90=113.61 mg/l after 24 hrs and LC50=48.20 mg/l and LC90=79.31 mg/l after 48 hrs at ?= 0.05. X 2 value was not significant at 24 h and is significant at 48 h. DMR test after 24 hrs. treatment, control and insecticide treated were significantly different from each while insecticide treated group has significantly higher than rest. After 48 hrs 4 and 5 treatment groups were found significantly higher than rest Larvicidal assay of A. indica has shown LC50 =52.36 mg/ l and LC90=105.42 mg/ l after 24 h and LC50 =80.70 mg/l and LC90=145.73 mg/ l after 48 h at ?= 0.05. X2 values for both 24h and 48h were not significant. DMR test after 24 hrs. insecticide treated was significantly lower than all above treatment groups. Treatment group 7 has significantly higher mortality than all other. After 48 hrs. there was not any significant difference. Lethal concentrations for L. camara LC50 =100.76 mg/100 ml and LC90=198.22 mg/100 ml at24 h and LC50 =61.27 mg/100 ml and LC90= 122.45mg/100 ml after 48 h at ?= 0.05. X2 value for both 24h and 48h was not significant. DMR test after 24 hrs. shows insecticide treated group i.e. 8 is significantly lower than all treatment groups except 1 which is at lowest treatment. After 48 4, 5 and 6 had came to same activity level no significant difference was found and were higher than all others. Conclusion: Hence it is concluded that crude plant extracts can act as potential Adulticide / Mosquitocide and Larvicides, though these are required in higher concentrations than that of synthetic insecticides and purified botanical products, but they have advantage of posssessing less resistance presentation and safe for aquatic life. It is also concluded that chemical insecticide used i.e. Deltamethrin has higher efficacy as adulticidal with drastic effects on environment and other beneficial insects. But it was found least effective as larvicidal compared to crude plants extracts. It is suggested that plant extracts can prove a better, safer and cheaper alternate these should consider as a better alternate to control most lethal disease of present time i.e. Dengue fever by eradicating its vector i.e. Aedes aegypti mosquito.
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Veterinary Science 1475,T (Browse shelf) Available 1475,T
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Pakistan is one of sub-tropical countries and its climatic conditions favor parasitic diseases along with disease vectors including mosquitoes. Mosquitoes of genus Aedes are causing Dengue fever. It has become one of infectious vector born disease of world. Annually it is striking around 100 million people with dengue fever and about 5 Lac people with Dengue Hemorrhagic Fever (DHF), resulting in 5 % deaths per year. Current study was conducted to evaluate efficacies of plant extracts against dengue fever mosquitoes of Aedes genus. Study target was to search some safer alternates than that of chemical pesticides. Also exhibiting characteristics like less toxic to environment, do not induce resistance in mosquitoes, do not harm non-targeted organisms, would not be toxic to human beings and will have biodegradation ability. Study has evaluated indigenous plants extracts as potent larvicidal and adulticidal materials.
Mosquitoes were collected from different areas of Lahore and they were identified upto genus level only Aedes genus was identified upto species level, then Aedes aegypti was reared in laboratory to have F 1 generation, to have mosquitoes and larvae for testing the activity of plant extracts. Plant materials were collected from different areas of Lahore. Methanolic extracts of seeds and leaves of Azadirachta indica and lantana camara respectively, were extracted accordingly. Stock solutions were prepared from these extracts and then out of this serial dilutions were made. Experiment was performed in four groups A, B, C & D for both adults and larvae. Each group was representing total of 25 larvae/adult out of which one was kept untreated
as negative control, one with deltamethrin as positive control while two were provided with test concentrations.

LC50 and LC90 was determined by Probit analysis, using SPSS version 13.0 SPSS inc. 2004. lethal concentrations of A. indica mosquitocidal assay were LC50=30.44 mg/l and LC90=62.36 mg/l after 24 hrs and LC50=-9.87mg/l and LC90=59.102 mg/l after 48 hrs at ?= 0.05. X 2 value was significant for 24 h while for 48 h it was not significant. Maximum mortality observed after 24 and 48 hrs is 99% at 70 ppm. DMR test shows after 24 hrs., all treatment means are significantlt different from each other, from control and from mean of insecticide's mortality, control is significantly lower while insecticide treated group has significantly higher than rest.
after 48 hrs. treatments were significantly different from each other while treatment mortality at 70 PPM is significantly higher than insecticide treated group and rest.
Lethal concentrations for L. camara adulticidal assay were LC50=60.40 mg/l and LC90=113.61 mg/l after 24 hrs and LC50=48.20 mg/l and LC90=79.31 mg/l after 48 hrs at ?= 0.05. X 2 value was not significant at 24 h and is significant at 48 h. DMR test after 24 hrs. treatment, control and insecticide treated were significantly different from each while insecticide treated group has significantly higher than rest. After 48 hrs 4 and 5 treatment groups were found significantly higher than rest
Larvicidal assay of A. indica has shown LC50 =52.36 mg/ l and LC90=105.42 mg/ l after 24 h and LC50 =80.70 mg/l and LC90=145.73 mg/ l after 48 h at ?= 0.05. X2 values for both 24h and 48h were not significant. DMR test after 24 hrs. insecticide treated was significantly lower than all above treatment groups. Treatment group 7 has significantly higher mortality than all other. After 48 hrs. there was not any significant difference.


Lethal concentrations for L. camara LC50 =100.76 mg/100 ml and LC90=198.22 mg/100 ml at24 h and LC50 =61.27 mg/100 ml and LC90= 122.45mg/100 ml after 48 h at ?= 0.05. X2 value for both 24h and 48h was not significant. DMR test after 24 hrs. shows insecticide treated group i.e. 8 is significantly lower than all treatment groups except 1 which is at lowest treatment. After 48 4, 5 and 6 had came to same activity level no significant difference was found and were higher than all others.
Conclusion:
Hence it is concluded that crude plant extracts can act as potential Adulticide / Mosquitocide and Larvicides, though these are required in higher concentrations than that of synthetic insecticides and purified botanical products, but they have advantage of posssessing less resistance presentation and safe for aquatic life. It is also concluded that chemical insecticide used i.e. Deltamethrin has higher efficacy as adulticidal with drastic effects on environment and other beneficial insects. But it was found least effective as larvicidal compared to crude plants extracts. It is suggested that plant extracts can prove a better, safer and cheaper alternate these should consider as a better alternate to control most lethal disease of present time i.e. Dengue fever by eradicating its vector i.e. Aedes aegypti mosquito.

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