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Mutagenic And Cytotoxic Evaluartion Of Piroxicam And Meloxicam

By: Snober Khatoon Akram | Prof. Dr. Muhammad Ashraf.
Contributor(s): Dr. Muhammad Adil Rasheed | Dr.Aftab | Faculty of Bio-Sciences.
Material type: materialTypeLabelBookPublisher: 2013Subject(s): Department of Pharmaoclogy & ToxicologyDDC classification: 1548,T Dissertation note: Piroxicam and Meloxicam are enolic acid derivatives and belong to oxicam class of non steroidal anti-inflammatory drugs. They are therapeutically used in rheumatoid arthritis and osteoarthritis. This study was designed to evaluate mutagenicity and cytotoxicity of piroxicam and meloxicam by Ames Salmonella/microsome mutagenicity assay and MTT assay. In this study, ten concentrations (100µg/ml, 300µg/ml, 500µg/ml, 700µg/ml, 900µg/ml, 1000µg/ml, 3000µg/ml, 5000µg/ml, 7000µg/ml and 10,000µg/ml) of piroxicam and meloxicam were used in Ames test against Salmonella strain TA100 in plate incorporation method, with and without metabolic activation S-9 mixture in triplicate manner. In MTT assay, confluent monolayer of BHK-21 cell lines was used and grown in 96-well cell culture plates treated with same concentrations of both drugs in triplicate manner. The results indicated that piroxicam had no mutagenic potential at concentrations of 100µg/plate to 3000µg/plate, possible mutagenic potential at 5000µg/plate and significant mutagenic potential at concentration of 7000µg/plate and 10,000µg/plate. Meloxicam had no mutagenic potential at the concentrations 100µg/plate to 7000µg/plate and possible mutagenic potential at highest concentration 10,000µg/plate. The cytotoxic effect of piroxicam and meloxicam at the concentrations of 100µg/ml to 5000µg/ml was none cytotoxic and at the concentration of 7000µg/ml and 10,000µg/ml cytotoxic to BHK-21 cell lines. There was significant increased in mutant frequency with increased in concentration of both drugs with and without metabolic activation S-9 mixture. There was significant difference in non mutagenic, possible mutagenic and significant mutagenic potential doses of piroxicam. There was no significant difference in none cytotoxic doses of both drugs. In comparison of both drugs, there was no significant difference in mutagenicity and cytotoxicity. It concluded that piroxicam and meloxicam were not mutagenic and cytotoxic at therapeutic doses. Piroxicam had mutagenic potential in dose dependent manner. Both drugs were cytotoxic at higher concentrations. They had same cytotoxic effect in dose dependent manner.
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Piroxicam and Meloxicam are enolic acid derivatives and belong to oxicam class of non steroidal anti-inflammatory drugs. They are therapeutically used in rheumatoid arthritis and osteoarthritis. This study was designed to evaluate mutagenicity and cytotoxicity of piroxicam and meloxicam by Ames Salmonella/microsome mutagenicity assay and MTT assay. In this study, ten concentrations (100µg/ml, 300µg/ml, 500µg/ml, 700µg/ml, 900µg/ml, 1000µg/ml, 3000µg/ml, 5000µg/ml, 7000µg/ml and 10,000µg/ml) of piroxicam and meloxicam were used in Ames test against Salmonella strain TA100 in plate incorporation method, with and without metabolic activation S-9 mixture in triplicate manner. In MTT assay, confluent monolayer of BHK-21 cell lines was used and grown in 96-well cell culture plates treated with same concentrations of both drugs in triplicate manner. The results indicated that piroxicam had no mutagenic potential at concentrations of 100µg/plate to 3000µg/plate, possible mutagenic potential at 5000µg/plate and significant mutagenic potential at concentration of 7000µg/plate and 10,000µg/plate. Meloxicam had no mutagenic potential at the concentrations 100µg/plate to 7000µg/plate and possible mutagenic potential at highest concentration 10,000µg/plate. The cytotoxic effect of piroxicam and meloxicam at the concentrations of 100µg/ml to 5000µg/ml was none cytotoxic and at the concentration of 7000µg/ml and 10,000µg/ml cytotoxic to BHK-21 cell lines. There was significant increased in mutant frequency with increased in concentration of both drugs with and without metabolic activation S-9 mixture. There was significant difference in non mutagenic, possible mutagenic and significant mutagenic potential doses of piroxicam. There was no significant difference in none cytotoxic doses of both drugs. In comparison of both drugs, there was no significant difference in mutagenicity and cytotoxicity. It concluded that piroxicam and meloxicam were not mutagenic and cytotoxic at therapeutic doses. Piroxicam had mutagenic potential in dose dependent manner. Both drugs were cytotoxic at higher concentrations. They had same cytotoxic effect in dose dependent manner.

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