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Evaluation Of Anti-Inflammatory And Analgesic Potential Of Aqueous Methanolic Extract Of Thuja Orientalis In Albino Rats

By: Muhammad Zahid Tanveer | Dr. Aqeel Javeed.
Contributor(s): Prof. Dr. Muhammad Ashraf.
Material type: materialTypeLabelBookPublisher: 2013Subject(s): Department of Pharmaoclogy & ToxicologyDDC classification: 1599,T Dissertation note: In the present study in vivo anti-inflammatory assay, central analgesic assay and peripheral analgesic estimation of methanolic extract of Thuja orientalis was performed by using carrageenan induced paw oedema model, hotplate test and acetic acid induced writhing test on albino rats, respectively. For anti-inflammatory assay, the experimental animals were divided into five groups each consisting of six animals and three groups of six animals were arranged each for central and peripheral analgesic evaluation. In all groups of animals in antiinflammatory assay, oedema was produced by using 0.1 ml of 1% carrageenan. The group II served as standard control group and was additionally treated with 10mg/Kg p.o indomethacin (a standard drug). The Groups III, IV and V received 50, 100 and 300 mg/Kg p.o of aqueous methanolic extract of Thuja orientalis (TO-Cr) respectively. All the treatment groups (II, III, IV and V) were treated 1 hour before injection of carrageenan. The volume of paw of rats was measured at 0 h and 3 h and the results of all treatment groups were compared with group I. In the present work, central analgesic study was done by using hot plate method. Tramadol was used as the standard drug in positive control group. Peripheral analgesia was determined by acetic induced writhing test using aspirin as standard analgesic drug. In the writhing test 1 % solution of acetic acid at dose of 0.1 ml / 10 grams was injected intra peritoneal. All the groups were pre treated 30 min before chemical stimulus with the standard drug and extract dose. Number of writhings was counted for 20 min. after injection. The statistical analysis of these values showed that results at 0 hour are non significant as P > 0.05 (Table 3).But it is evaluated from the study of paw volumes after 3 hours that there was significant decrease in oedema in group treated with standard drug i.e. indomethacin (79.70 % decrease) as compared with the 60 negative control (Fig. 11). The response of the extract under study was dose related. There was 13 % decrease in paw oedema as compared with negative control at 50 mg / kg dose of TO-Cr (Table 7). Similarly there was 34 % and 59.57 % decrease in paw oedema as compared with negative control at 100 mg / kg and 300 mg / kg doses of TO-Cr (Table 7). In central analgesic model of hotplate, there was significant increase in latency time in treatment group at 60 min interval (Table 15) and then it remained almost same after 90 min (Table 18). In peripheral analgesia of acetic acid induced writhing test, there was significant decrease in the number of writhings in positive control (7.33+1.63) and Thuja orientalis extract (12.50+2.35) also decreased the number of writhings significantly as compared with the negative control group (20.67+2.16) (Table 22). It is concluded from the results that aqueous methanolic extract of the fruit of Thuja orientalis has significant anti-inflammatory activity and produced dose dependant reduction in inflammation and it also has both central and peripheral analgesic properties.
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In the present study in vivo anti-inflammatory assay, central analgesic assay and
peripheral analgesic estimation of methanolic extract of Thuja orientalis was performed by
using carrageenan induced paw oedema model, hotplate test and acetic acid induced writhing
test on albino rats, respectively. For anti-inflammatory assay, the experimental animals were
divided into five groups each consisting of six animals and three groups of six animals were
arranged each for central and peripheral analgesic evaluation. In all groups of animals in antiinflammatory
assay, oedema was produced by using 0.1 ml of 1% carrageenan. The group II
served as standard control group and was additionally treated with 10mg/Kg p.o indomethacin
(a standard drug). The Groups III, IV and V received 50, 100 and 300 mg/Kg p.o of aqueous
methanolic extract of Thuja orientalis (TO-Cr) respectively. All the treatment groups (II, III, IV
and V) were treated 1 hour before injection of carrageenan. The volume of paw of rats was
measured at 0 h and 3 h and the results of all treatment groups were compared with group I. In
the present work, central analgesic study was done by using hot plate method. Tramadol was
used as the standard drug in positive control group. Peripheral analgesia was determined by
acetic induced writhing test using aspirin as standard analgesic drug. In the writhing test 1 %
solution of acetic acid at dose of 0.1 ml / 10 grams was injected intra peritoneal. All the groups
were pre treated 30 min before chemical stimulus with the standard drug and extract dose.
Number of writhings was counted for 20 min. after injection. The statistical analysis of these
values showed that results at 0 hour are non significant as P > 0.05 (Table 3).But it is evaluated
from the study of paw volumes after 3 hours that there was significant decrease in oedema in
group treated with standard drug i.e. indomethacin (79.70 % decrease) as compared with the
60
negative control (Fig. 11). The response of the extract under study was dose related. There was
13 % decrease in paw oedema as compared with negative control at 50 mg / kg dose of TO-Cr
(Table 7). Similarly there was 34 % and 59.57 % decrease in paw oedema as compared with
negative control at 100 mg / kg and 300 mg / kg doses of TO-Cr (Table 7). In central analgesic
model of hotplate, there was significant increase in latency time in treatment group at 60 min
interval (Table 15) and then it remained almost same after 90 min (Table 18). In peripheral
analgesia of acetic acid induced writhing test, there was significant decrease in the number of
writhings in positive control (7.33+1.63) and Thuja orientalis extract (12.50+2.35) also
decreased the number of writhings significantly as compared with the negative control group
(20.67+2.16) (Table 22).
It is concluded from the results that aqueous methanolic extract of the fruit of Thuja
orientalis has significant anti-inflammatory activity and produced dose dependant reduction in
inflammation and it also has both central and peripheral analgesic properties.

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