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Bioconversion Of Whey To Beta-Galactosidase By Aspergillus Niger

By: Muhammad Tayyab Younas | Ms. Huma Mujahid.
Contributor(s): Dr. Abu Saeed Hashmi | Ms. Sehrish.
Material type: materialTypeLabelBookPublisher: 2013Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1387,T Dissertation note: Beta-galactosidase (lactase) has catalytic property to hydrolyze lactose into glucose and galactose. It is extensively used for the synthesis of milk made products through fermentation. Food rich in lactose have variety of application in industrial and environmental processes. In present study production, purification andcharacterization of ?-galactosidase synthesized by Aspergillus niger has been considered as a great challenge. Beta-glactosidase is an important enzyme involved in conversion of lactose into glucose and galactose and produced on industrial scale for its large applications in the field of health, and food. The production of beta-galactosidase was carried out from fungal culture of Aspergillus niger using whey as a substrate. Optimization of different physical parameters such as temperature, pH, addition of corn steep liquor and production, purification and characterization of beta galactosidase enzyme from Aspergillus niger were studied. Optimum concentration of whey (4mL) were found 13.42 IU/mL and activity of beta galactosidase was found maximum at 72 h of incubation period and further incubation period decline the activity.Optimum pH (13.50 IU/mL)and temperature (17.67 IU/mL) were found 5.5 and 40°C respectively. Addition of corn steep liquor was enhanced the activity of beta galactosidase. Maximum activity was found with 0.6% of corn steep liquor which was 19.4IU/mLas compare to the other nitrogen sources. Finally, addition of ammonium sulphate ?-galactosidase was purified. ?-galactosidase was characterized considering ortho-Nitrophenyl-?-galactoside (ONPG) and whey as a substrate The purified beta-galactosidase was confirmed by SDS PAGE analysis which has molecular weight of 74kDa. The study could also establish that whey could effectively be utilized for ?- galactosidase production thus alleviating water pollution problems caused due to its disposal into the water streams.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
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Veterinary Science 1387,T (Browse shelf) Available 1387,T
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Beta-galactosidase (lactase) has catalytic property to hydrolyze lactose into glucose and galactose. It is extensively used for the synthesis of milk made products through fermentation. Food rich in lactose have variety of application in industrial and environmental processes.
In present study production, purification andcharacterization of ?-galactosidase synthesized by Aspergillus niger has been considered as a great challenge. Beta-glactosidase is an important enzyme involved in conversion of lactose into glucose and galactose and produced on industrial scale for its large applications in the field of health, and food. The production of beta-galactosidase was carried out from fungal culture of Aspergillus niger using whey as a substrate. Optimization of different physical parameters such as temperature, pH, addition of corn steep liquor and production, purification and characterization of beta galactosidase enzyme from Aspergillus niger were studied. Optimum concentration of whey (4mL) were found 13.42 IU/mL and activity of beta galactosidase was found maximum at 72 h of incubation period and further incubation period decline the activity.Optimum pH (13.50 IU/mL)and temperature (17.67 IU/mL) were found 5.5 and 40°C respectively. Addition of corn steep liquor was enhanced the activity of beta galactosidase. Maximum activity was found with 0.6% of corn steep liquor which was 19.4IU/mLas compare to the other nitrogen sources. Finally, addition of ammonium sulphate ?-galactosidase was purified. ?-galactosidase was characterized considering ortho-Nitrophenyl-?-galactoside (ONPG) and whey as a substrate The purified beta-galactosidase was confirmed by SDS PAGE analysis which has molecular weight of 74kDa. The study could also establish that whey could effectively be utilized for ?- galactosidase production thus alleviating water pollution problems caused due to its disposal into the water streams.

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