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Isolation, Identification And Control Of Vancomycin Resistant Staphylococcus Aureus

By: Fakhra Liaqat | Dr. Ali Ahmad Sheikh.
Contributor(s): Dr. Jawad Nazir | Dr. Tanveer Hussain.
Material type: materialTypeLabelBookPublisher: 2013Subject(s): Department of MicrobiologyDDC classification: 1615,T Dissertation note: Antimicrobial resistance (AMR) is an emerging issue throughout the world. Vancomycin resistant Staphylococcus aureus has been recently reported from many countries including Asian region. The failure of antibiotics diverts the focus of modern science towards the discovery and application of new alternative antimicrobial agents. Herbal plants not only known for their antimicrobial potential but are being used since centuries for the treatment of infections. This study has been conducted to isolate Vancomycin Resistant Staphylococcus aureus from wounds of hospitalized patients and these isolates were not only tested against Linezolid, Moxifloxacin, and Clindamycin antibiotics but also against the ethanolic extracts of Garlic, Mint, Coriander, Turmeric, Kalonji, Cinnamon and Cloves for their antibacterial activity. Methicillin resistant Staphylococcus aureus were isolated from wounds and resistance to vancomycin was confirmed according to Clinical and Laboratory Standards Institute recommended method. Minimum inhibitory concentrations of selected antibiotics and selected plant extract was determined by broth microdilution method followed by the measurement of minimum bactericidal concentration by culturing on agar plates. In current study 104 S. aureus isolates were recovered from 150 wound exudates samples. Resistance to methicillin was shown by 49.04% isolates. Final results yielded, 22 vancomycin intermediate and 5 vancomycin resistant S. aureus strains. Vancomycin resistant isolates were tested for susceptibility against selected antibiotics and ethanolic extracts of plants. Almost all the isolates displayed susceptibility to all three antibiotics and the plant extracts. The data was analyzed statistically by chi square test and one way ANOVA using Statistical Package for Social Science (SPSS) 18.0 software. This study was helpful to find out the effective antibiotics against Vancomycin Resistant Staphylococcus aureus. Plant extracts which were found effective are the best alternate to the conventional antibiotics without having any drawback of antibiotic resistance development.
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Antimicrobial resistance (AMR) is an emerging issue throughout the world. Vancomycin resistant Staphylococcus aureus has been recently reported from many countries including Asian region. The failure of antibiotics diverts the focus of modern science towards the discovery and application of new alternative antimicrobial agents. Herbal plants not only known for their antimicrobial potential but are being used since centuries for the treatment of infections. This study has been conducted to isolate Vancomycin Resistant Staphylococcus aureus from wounds of hospitalized patients and these isolates were not only tested against Linezolid, Moxifloxacin, and Clindamycin antibiotics but also against the ethanolic extracts of Garlic, Mint, Coriander, Turmeric, Kalonji, Cinnamon and Cloves for their antibacterial activity.
Methicillin resistant Staphylococcus aureus were isolated from wounds and resistance to vancomycin was confirmed according to Clinical and Laboratory Standards Institute recommended method. Minimum inhibitory concentrations of selected antibiotics and selected plant extract was determined by broth microdilution method followed by the measurement of minimum bactericidal concentration by culturing on agar plates.
In current study 104 S. aureus isolates were recovered from 150 wound exudates samples. Resistance to methicillin was shown by 49.04% isolates. Final results yielded, 22 vancomycin intermediate and 5 vancomycin resistant S. aureus strains. Vancomycin resistant isolates were tested for susceptibility against selected antibiotics and ethanolic extracts of plants. Almost all the isolates displayed susceptibility to all three antibiotics and the plant extracts. The data was analyzed statistically by chi square test and one way ANOVA using Statistical Package for Social Science (SPSS) 18.0 software. This study was helpful to find out the effective antibiotics against Vancomycin Resistant Staphylococcus aureus. Plant extracts which were found effective are the best alternate to the conventional antibiotics without having any drawback of antibiotic resistance development.

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