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Detection of Insulin-Like Growth Factor-I (IGF-I) Gene Polymorphism in Native Aseel, Desi and Naked Neck Chicken Breeds in Pakistan.

By: Asad Ali | Prof. Dr. Khalid Javed.
Contributor(s): Dr. Afzal Ali | Prof. Dr. Muhammad.
Material type: materialTypeLabelBookPublisher: 2013Subject(s): Department of Animal NutritionDDC classification: 1637,T Dissertation note: Growth traits in chicken production system have an important role. Molecular analysis is an easier mean to identify desirable genotypes for growth. Candidate gene (s) for growth trait like insulin-like growth factor-1 (IGF-1) has imperative function for growth, body composition, metabolic and skeletal traits. The polymorphism of Insulin-like growth factor-1 was detected in native Aseel, Desi and Naked Neck chicken breeds of Pakistan. Fifty, fifty birds of Aseel and Naked Neck breed were selected from Indigenous chicken genetic resource center, Department of Poultry Production, UVAS, Lahore, Ravi Campus. While Desi birds were procured from conventional production farm for genetic analysis. Insulin-like growth factor-1 plays very important role in the cell growth, cell differentiation, food intake of cells and have role in overall body growth, this is why it is being used as a marker to study traits like growth. The polymorphism of IGF-I gene was detected by PCR-RFLP-Pst-Iand this revealed two alleles A (364 and 257 bp), B (621 bp),and three genotypes AA, AB, BB.Genotypic data was analyzed with the help of Pop-gene 1.32 software to calculate genotypic and allelic frequencies. GenotypeAB had the highest frequency in all three native breeds. Genotypic frequency of AA, AB and BB in Aseel was 20, 66 and 14% and in Desi it was 12, 64 and 24 % respectively. While in Naked Neck genotypic frequencies of AA, AB and BB were 18, 60 and 22 %. The highest frequency of allele A (0.53) was found in Aseel while highest frequency of Allele B (0.56) was found in Desi. Genotypic frequency of heterozygotes was highest in all three native breeds i.e. 66, 64 and 60 % in Aseel, Desi and Naked Neck respectively. As the frequency of heterozygotes is significantly higher in all three breeds, so all these native breeds have potential to improve through selection.Furtherstudies are needed to link these polymorphisms with performance traits and then using that information in future breeding plans for high producing individuals would be very helpful for animal breeder in marker assisted selection (MAS). As Aseel carried highest frequency of allele A so, it can be reared for selective breeding program especially for meat type line development in Pakistan.
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Growth traits in chicken production system have an important role. Molecular analysis is an easier mean to identify desirable genotypes for growth. Candidate gene (s) for growth trait like insulin-like growth factor-1 (IGF-1) has imperative function for growth, body composition, metabolic and skeletal traits. The polymorphism of Insulin-like growth factor-1 was detected in native Aseel, Desi and Naked Neck chicken breeds of Pakistan. Fifty, fifty birds of Aseel and Naked Neck breed were selected from Indigenous chicken genetic resource center, Department of Poultry Production, UVAS, Lahore, Ravi Campus. While Desi birds were procured from conventional production farm for genetic analysis. Insulin-like growth factor-1 plays very important role in the cell growth, cell differentiation, food intake of cells and have role in overall body growth, this is why it is being used as a marker to study traits like growth. The polymorphism of IGF-I gene was detected by PCR-RFLP-Pst-Iand this revealed two alleles A (364 and 257 bp), B (621 bp),and three genotypes AA, AB, BB.Genotypic data was analyzed with the help of Pop-gene 1.32 software to calculate genotypic and allelic frequencies. GenotypeAB had the highest frequency in all three native breeds. Genotypic frequency of AA, AB and BB in Aseel was 20, 66 and 14% and in Desi it was 12, 64 and 24 % respectively. While in Naked Neck genotypic frequencies of AA, AB and BB were 18, 60 and 22 %. The highest frequency of allele A (0.53) was found in Aseel while highest frequency of Allele B (0.56) was found in Desi. Genotypic frequency of heterozygotes was highest in all three native breeds i.e. 66, 64 and 60 % in Aseel, Desi and Naked Neck respectively. As the frequency of heterozygotes is significantly higher in all three breeds, so all these native breeds have potential to improve through selection.Furtherstudies are needed to link these polymorphisms with performance traits and then using
that information in future breeding plans for high producing individuals would be very helpful for animal breeder in marker assisted selection (MAS). As Aseel carried highest frequency of allele A so, it can be reared for selective breeding program especially for meat type line development in Pakistan.

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