Cytogenetic Analysis Related To Infertity Problems In Buffalo In Pakistan
By: Muhammad Dawood | Prof. Dr. khalid Javaid.
Contributor(s): Prof. Dr. masroor Ellahi babar.
Material type: BookPublisher: 2013Subject(s): Department of Livestock ProductionDDC classification: 1699,T Dissertation note: Cytogenetics is a branch of genetics which deals with the study of the structure and function of the cell, especially the chromosomes. It includes routine analysis of chromosomes using different banding techniques and fluorescent in situ hybridization (FISH). Chromosomes were first discovered in plant cells by Karl Wilhelm von Nägeli in 1842 and in animal (salamander) cells were described by Walther Flemmin, in 1882. In 1964 IngemarGustavsson was the first who work on clinical cytogenetics in animals and found first 1/29 robertsonian translocation in cattle. Hundreds of abnormalities have been reported in past 50 years with clinical disorders. Some abnormalities include robertsonian translocation in captive Thai Gaur (Chaveerach et al. 2007), in Veitnamese cattle (Tanaka et al. 2000), pericentric inversion of chromosomes and chimeric karyotypes in cattle and buffalo, sex chromosomes reciprocal translocation including X;X translocation in Mehsana buffalo (Patel et al. 2006) and bovine freemartin syndrome. Livestock population have been extraordinarily improved in the last 40 years through cytogenetic screening (Ducos et al. 2008). This study was carried out to optimize the protocol for lymphocyte culture, harvesting and slide preparation techniques for indigenous buffalo in local conditions and to document the incidence of chromosomal abnormalities leading to infertility problems in buffalo. Lymphocyte culture was used for the cytogenetic studies of indigenous buffalo. Procedure for lymphocyte and culture was standardized in Animal Genetic Lab, University of Veterinary and Animal Sciences, Ravi Campus. In blood cell culture incubation time will be 72 hours at 370C. Mitosis was induced through Phytohaemagglutinin. After incubation cells were arrested by colcimid. Slides were prepared for karyotyping and checking for the chromosomal abnormalities. In this study 30 repeat breeder and anestrus animals along with 5 normal animals were used for chromosomal analysis. Good quality metaphase spreads were cytogenetically analyzed for chromosomal abnormalities (structural and numerical). Results of this study show that there is not any sex chromosome abnormalities and autosomal abnormality foundin the group of repeat breeders and anestrus animals. All the standard procedures regarding cytogenetic culture, harvesting, slide preparation and staining were optimized in Animal Genetic Lab. Genus 3.2 Applied Imaging System software was used for karyotyping the animals. We can conclude that cytogenetic analysis can play more effective role in the betterment of livestock. Through this technique we can screen out abnormal animals at very early stage.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
---|---|---|---|---|---|---|---|
Thesis | UVAS Library Thesis Section | Veterinary Science | 1699,T (Browse shelf) | Available | 1699,T |
Cytogenetics is a branch of genetics which deals with the study of the structure and function of the cell, especially the chromosomes. It includes routine analysis of chromosomes using different banding techniques and fluorescent in situ hybridization (FISH). Chromosomes were first discovered in plant cells by Karl Wilhelm von Nägeli in 1842 and in animal (salamander) cells were described by Walther Flemmin, in 1882.
In 1964 IngemarGustavsson was the first who work on clinical cytogenetics in animals and found first 1/29 robertsonian translocation in cattle. Hundreds of abnormalities have been reported in past 50 years with clinical disorders. Some abnormalities include robertsonian translocation in captive Thai Gaur (Chaveerach et al. 2007), in Veitnamese cattle (Tanaka et al. 2000), pericentric inversion of chromosomes and chimeric karyotypes in cattle and buffalo, sex chromosomes reciprocal translocation including X;X translocation in Mehsana buffalo (Patel et al. 2006) and bovine freemartin syndrome. Livestock population have been extraordinarily improved in the last 40 years through cytogenetic screening (Ducos et al. 2008).
This study was carried out to optimize the protocol for lymphocyte culture, harvesting and slide preparation techniques for indigenous buffalo in local conditions and to document the incidence of chromosomal abnormalities leading to infertility problems in buffalo. Lymphocyte culture was used for the cytogenetic studies of indigenous buffalo. Procedure for lymphocyte and culture was standardized in Animal Genetic Lab, University of Veterinary and Animal Sciences, Ravi Campus. In blood cell culture incubation time will be 72 hours at 370C. Mitosis was induced through Phytohaemagglutinin. After incubation cells were arrested by colcimid. Slides were prepared for karyotyping and checking for the chromosomal abnormalities.
In this study 30 repeat breeder and anestrus animals along with 5 normal animals were used for chromosomal analysis. Good quality metaphase spreads were cytogenetically analyzed for chromosomal abnormalities (structural and numerical). Results of this study show that there is not any sex chromosome abnormalities and autosomal abnormality foundin the group of repeat breeders and anestrus animals.
All the standard procedures regarding cytogenetic culture, harvesting, slide preparation and staining were optimized in Animal Genetic Lab. Genus 3.2 Applied Imaging System software was used for karyotyping the animals.
We can conclude that cytogenetic analysis can play more effective role in the betterment of livestock. Through this technique we can screen out abnormal animals at very early stage.
There are no comments for this item.