Molecular Characterization Of Antimicrobial Resistance Genes In Salmonella Isolates From Diarrheic Calves
By: Hania Zulfiqar
| Prof. Dr. Tahir Yaqub.
Contributor(s): Dr. Abu Saeed | Miss. Sehrish Firyal.
Material type: 
BookPublisher: 2013Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1744,T Dissertation note: A number of infectious (bacteria, viruses, parasites) and non-infectious factors cause diarrhoea in calves. Salmonella bacteria are gram-negative and belong to the family Enterobacteriaceae. Salmonella infections in calves continue to be a major problem worldwide and are responsible of causing major economical losses. To avoid the consequences of disease caused by Salmonella drugs like pencillin, tetracyclines e.g, are given to cattle but it is observed that Salmonella show resistance against these drugs after certain period of time. Salmonella is the major causative agent of calf diarrhea. The antibiotic genes against tetracycline and ampicillin are present in slamonella isolates from calves which are suffering from diarrhea.
Aim of my study was 1) Salmonella isolation and investigation of the antimicrobial resistance gene from diarrheic calves and 2) Molecular analysis of antibiotic resistance gene of isolated salmonella species. For this purpose, salmonella antibiotic resistant isolates against ampicillin and tetracycline were selected. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer.
After sequencing all the sequences were viewed in Chromas Lite 2.1.1 , Sample sequences were aligned with the reference sequences obtained from NCBI by using Mega 5.05 software. Alignment results show that there is no Single Nucleotide Polymorphism found in salmonella.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1744,T (Browse shelf) | Available | 1744,T |
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A number of infectious (bacteria, viruses, parasites) and non-infectious factors cause diarrhoea in calves. Salmonella bacteria are gram-negative and belong to the family Enterobacteriaceae. Salmonella infections in calves continue to be a major problem worldwide and are responsible of causing major economical losses. To avoid the consequences of disease caused by Salmonella drugs like pencillin, tetracyclines e.g, are given to cattle but it is observed that Salmonella show resistance against these drugs after certain period of time. Salmonella is the major causative agent of calf diarrhea. The antibiotic genes against tetracycline and ampicillin are present in slamonella isolates from calves which are suffering from diarrhea.
Aim of my study was 1) Salmonella isolation and investigation of the antimicrobial resistance gene from diarrheic calves and 2) Molecular analysis of antibiotic resistance gene of isolated salmonella species. For this purpose, salmonella antibiotic resistant isolates against ampicillin and tetracycline were selected. Antibiotic resistant plasmid genes were amplified by PCR. After gel electrophoresis the resulting fragments were sequenced through genetic analyzer.
After sequencing all the sequences were viewed in Chromas Lite 2.1.1 , Sample sequences were aligned with the reference sequences obtained from NCBI by using Mega 5.05 software. Alignment results show that there is no Single Nucleotide Polymorphism found in salmonella.
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