Vectorial Role Of Anopheles Subpictus By Using Poymerase Chain Reaction
By: Shumaila Kausar
| Prof. Dr. Kamran Ashraf.
Contributor(s): Dr. Aftab | Dr. Haroon Akber.
Material type: 
BookPublisher: 2014Subject(s): Department of ParasitologyDDC classification: 1811,T Dissertation note: Vector-borne diseases are among the major causes of illness, death and economic losses particularly in tropical and subtropical areas of the world. Mosquitoes are most important single group of insects, transmitting wide range of human diseases like malaria, Japanese encephalitis, dengue fever, yellow fever, filariasis and several other infectious diseases. Malaria is a major global public health problem. Several Anopheline species are prevalent in Pakistan among which Anopheles stephensi and Anopheles culicifacies are confirmed vector of Plasmodium. An. subpictus is a confirmed secondary vector of malaria in many countries. A wide range of this species is also distributed in Pakistan. The aim of the present study was to determine the vectorial role of An. subpictus in the transmission of malaria in Pakistan through microscopy and polymerase chain reaction (PCR).
After species identification, the mosquitoes were examined microscopically and were found negative for the presence of human Plasmodium sporozoites. The DNA extracted from An. subpictus was subjected to PCR but no Plasmodium specific amplification was observed. Keeping in view the sample size, it was concluded that further study is required with even large sample size before declaring that An. subpictus is not a vector of Plasmodium in Pakistan.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1811,T (Browse shelf) | Available | 1811,T |
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Vector-borne diseases are among the major causes of illness, death and economic losses particularly in tropical and subtropical areas of the world. Mosquitoes are most important single group of insects, transmitting wide range of human diseases like malaria, Japanese encephalitis, dengue fever, yellow fever, filariasis and several other infectious diseases. Malaria is a major global public health problem. Several Anopheline species are prevalent in Pakistan among which Anopheles stephensi and Anopheles culicifacies are confirmed vector of Plasmodium. An. subpictus is a confirmed secondary vector of malaria in many countries. A wide range of this species is also distributed in Pakistan. The aim of the present study was to determine the vectorial role of An. subpictus in the transmission of malaria in Pakistan through microscopy and polymerase chain reaction (PCR).
After species identification, the mosquitoes were examined microscopically and were found negative for the presence of human Plasmodium sporozoites. The DNA extracted from An. subpictus was subjected to PCR but no Plasmodium specific amplification was observed. Keeping in view the sample size, it was concluded that further study is required with even large sample size before declaring that An. subpictus is not a vector of Plasmodium in Pakistan.
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