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Reversal Of Antibiotics Resistance In Methicillin-Resistant Staphylococcus Aureus By Non-Antimicrobial Agents

By: Sohaib Danyaal | Prof. Dr. Muhammad Ashraf.
Contributor(s): Dr. Aftab | Dr. Muhammad Adil Rasheed.
Material type: materialTypeLabelBookPublisher: 2014Subject(s): Department of Pharmaoclogy & ToxicologyDDC classification: 1828,T Dissertation note: Antibiotic resistance is increasing along with the increasing use of antibiotic for the treatment of infectious diseases. The in-vitro study was designed to observe the reversal of antibiotics resistance in Methicillin-resistant Staphylococcus aureus by non-antimicrobial agents. One hundred pus samples were processed for isolation and identification of MRSA. Out of 100, 37 (37%) islotes were Gram +ve cocci, from these 37 isolates 34 (92%) turn red colour of mannitol salt media to yellow and 23 (62%) gave +ve catalase and coagulase. In this study Antibiotic Sensitivity Test was performed on pure culture of MRSA strain by applying disc diffusion method. Out of 23 pure MRSA isolate, 100% isolates were methacillin resistant, 79% isolates were co-amoxiclav resistant, 30% isolates were meropenum resistant, 8% isolates were vancomycin resistant, 26% isoltes were moxifloxacin resistant and 39% isolates were linezolid resistant. Reversal of antibiotics resistance was observed by MIC or serial dilution method, using non antibiotic agents like Amiloride, Lansoprazole and Promethazine, Concentrations of non-antibiotic agents 1024ìg, 512ìg, 256ìg, 128ug, 64ìg and 32ìg were used in combination of antibiotics to reverse the antibiotics resistance in MRSA. These non antibiotic agents may cause the alteration in mechanisms by which microorganism develop resistance. The collected data analyzed by applying analysis of variance (ANOVA) through SPSS 16.0 computer software. Now we would be able to treat some lethal infection caused by MRSA, and help to increase patient compliance and decrease the cost of therapy.
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Antibiotic resistance is increasing along with the increasing use of antibiotic for the treatment of infectious diseases. The in-vitro study was designed to observe the reversal of
antibiotics resistance in Methicillin-resistant Staphylococcus aureus by non-antimicrobial
agents.
One hundred pus samples were processed for isolation and identification of MRSA.
Out of 100, 37 (37%) islotes were Gram +ve cocci, from these 37 isolates 34 (92%) turn red
colour of mannitol salt media to yellow and 23 (62%) gave +ve catalase and coagulase. In
this study Antibiotic Sensitivity Test was performed on pure culture of MRSA strain by
applying disc diffusion method. Out of 23 pure MRSA isolate, 100% isolates were
methacillin resistant, 79% isolates were co-amoxiclav resistant, 30% isolates were
meropenum resistant, 8% isolates were vancomycin resistant, 26% isoltes were moxifloxacin
resistant and 39% isolates were linezolid resistant. Reversal of antibiotics resistance was
observed by MIC or serial dilution method, using non antibiotic agents like Amiloride,
Lansoprazole and Promethazine, Concentrations of non-antibiotic agents 1024ìg, 512ìg,
256ìg, 128ug, 64ìg and 32ìg were used in combination of antibiotics to reverse the
antibiotics resistance in MRSA. These non antibiotic agents may cause the alteration in
mechanisms by which microorganism develop resistance. The collected data analyzed by
applying analysis of variance (ANOVA) through SPSS 16.0 computer software. Now we would be
able to treat some lethal infection caused by MRSA, and help to increase patient compliance
and decrease the cost of therapy.

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