Real-Time PCR : An Essential Guide
By: Edwards, Kirsten.
Contributor(s): Logan, Julie | Saunders, Nick.
Material type: BookPublisher: UK: Taylor & Francis; 2004Edition: 1st ed.Description: 300 p.ISBN: 095452327X (hardcover); 9780954523275 (hardcover).Subject(s): Clinical Microbiology | Polymerase chain reaction (PCR) | Diagnosis of Infection | DNADDC classification: 572.43 Kirsten 17237 1st 2004 Microbiology Summary: The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. This essential manual presents a comprehensive guide to the most appropriate and up-to-date technologies and applications as well as providing an overview of the theory of this important technique. Written by recognized experts in the field this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: Real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Books | UVAS Library Microbiology | Veterinary Science | 572.43 Kirsten 17237 1st 2004 Microbiology (Browse shelf) | Available | 17237 |
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The polymerase chain reaction (PCR) is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. This essential manual presents a comprehensive guide to the most appropriate and up-to-date technologies and applications as well as providing an overview of the theory of this important technique. Written by recognized experts in the field this timely and authoritative volume is an essential requirement for all laboratories using PCR. Topics covered include: Real-time PCR instruments and probe chemistries, set-up, controls and validation, quantitative real-time PCR, analysis of mRNA expression, mutation detection, NASBA, application in clinical microbiology and diagnosis of infection.
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