PRINS and In Situ PCR Protocols
By: Pellestor, Franck
.
Material type: 
BookSeries: Publisher: USA: Humana Press; 2006Edition: 2nd ed.Description: 244 p.ISBN: 1588295494 (hardcover); 9781588295491 (hardcover).Subject(s): Polymerase chain reaction (PCR) | Diagnosis of Infection | DNA | Clinical Microbiology | In Situ HybridizationDDC classification: 572.84 Frank 19013 2nd 2006 Microbiology Summary: The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Books
|
UVAS Library Microbiology | Veterinary Science | 572.84 Frank 19013 2nd 2006 Microbiology (Browse shelf) | Available | 19013 |
Browsing UVAS Library Shelves , Shelving location: Microbiology , Collection code: Veterinary Science Close shelf browser
| 572.43 Domingues 32670 1st 2017 Microbiology PCR: Methods and Protocols | 572.43 Kirsten 17237 1st 2004 Microbiology Real-Time PCR : An Essential Guide | 572.803 Singleton 15302 3rd 2002 Microbiology Dictionary of Microbiology and Molecular Biology / 3rd ed | 572.84 Frank 19013 2nd 2006 Microbiology PRINS and In Situ PCR Protocols | 572.86 Philip 32324 1st 2016 Microbiology Microbial Engineering: Concepts and Applications | 574 Campbell 24019 8th 2008 Microbiology Biology / 8th ed | 574.23 Pedro 12847 1st 1977 Microbiology The Specificity And Action Of Animal, Bacterial, And Plant Toxins |
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
There are no comments for this item.