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Production Of Synbiotic Product Containing Galacto-Oligosaccharides And Saccharomyces Boulardii And Evaluation Of Its In Vitro Bifidogenic Effect

By: Farah Javed (2013-Mphil-1110) | Dr. Sanaullah Iqbal.
Contributor(s): Dr. Zubair Farooq | Miss Faiza Masood.
Material type: materialTypeLabelBookPublisher: 2015Description: 57p.Subject(s): Department of Food and NutritionDDC classification: 2291-T Dissertation note: Food are not only used to satisfy hunger or provide nutritional support but also used to prevent diseases that are based on nutrition and thus improve the health of consumers (Menrad, 2003).A food that is same in appearance and consumption to conventional food but has physiological benefits and prevents diseases is known as functional food (Sangwan et al. 2011). Functional foods are used to provide benefit to intestinal microflora. The most important functional food are prebiotic and probiotic. The combination of prebiotic and probiotic are known as synbiotics. The symbiotic is effective as that of prebiotic and probiotic. The probiotic is used to reduce the impurities from prebiotic mixture. This is very important to know the functional property of functional food (Sangwan et al. 2014) that is galactooligosaccharides (GOS) in present research. GOS are most effective prebiotic which are beneficial to intestinal microbiota. Other health effects are stool improvement, mineral absorption, weight management and also improve the growth of Bifidobacterium and Lactobacillus (Macfarlane et al. 2008). The Saccharomyces boulardii is a non-pathogenic yeast and used as probiotic. It is thermo tolerant yeast and optimally grows at body temperature i.e; 37ºC. Due to this property S. boulardii can be used for the treatment of gastrointestinal diseases. Another function of this yeast is the reduction of monosaccharides from prebiotic mixture. The purposes of present research work are to reduce the monosaccharides form mixture of GOS and the production of synbiotic product containing GOS and S. boulardii. For this GOS were produced through transgalactosylation procedure using lactose as substrate. S. boulardii was grown separately and incubated with the mixture of GOS. After that symbiotic product was formed and lyophilized it. The total count of yeast was determined in lyophilized mixture. The in vitro bifidogenic effect of GOS and lyophilized product was also checked.
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Food are not only used to satisfy hunger or provide nutritional support but also used to prevent diseases that are based on nutrition and thus improve the health of consumers (Menrad, 2003).A food that is same in appearance and consumption to conventional food but has physiological benefits and prevents diseases is known as functional food (Sangwan et al. 2011). Functional foods are used to provide benefit to intestinal microflora. The most important functional food are prebiotic and probiotic. The combination of prebiotic and probiotic are known as synbiotics. The symbiotic is effective as that of prebiotic and probiotic. The probiotic is used to reduce the impurities from prebiotic mixture. This is very important to know the functional property of functional food (Sangwan et al. 2014) that is galactooligosaccharides (GOS) in present research.
GOS are most effective prebiotic which are beneficial to intestinal microbiota. Other health effects are stool improvement, mineral absorption, weight management and also improve the growth of Bifidobacterium and Lactobacillus (Macfarlane et al. 2008). The Saccharomyces boulardii is a non-pathogenic yeast and used as probiotic. It is thermo tolerant yeast and optimally grows at body temperature i.e; 37ºC. Due to this property S. boulardii can be used for the treatment of gastrointestinal diseases. Another function of this yeast is the reduction of monosaccharides from prebiotic mixture.
The purposes of present research work are to reduce the monosaccharides form mixture of GOS and the production of synbiotic product containing GOS and S. boulardii. For this GOS

were produced through transgalactosylation procedure using lactose as substrate. S. boulardii was grown separately and incubated with the mixture of GOS. After that symbiotic product was formed and lyophilized it. The total count of yeast was determined in lyophilized mixture. The in vitro bifidogenic effect of GOS and lyophilized product was also checked.

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