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Efficacy Of Surface Disinfectants Against Bacterial Pathogens On Experimentally Contaminated Pathogens

By: Sana Ahmed (2009-VA-241) | Dr. Jawad Nazir.
Contributor(s): Dr. Amir Ghafoor | Prof. Dr. Tahir Yaqub.
Material type: materialTypeLabelBookPublisher: 2015Description: 62p.Subject(s): Department of MicrobiologyDDC classification: 2294-T Dissertation note: Surface disinfection plays a key role in the prevention, control and reduction of many communicable infections as contaminated surfaces are the major source for transmission of microorganisms. Five types of surface cleaning products (Astonish Germ Clear, Cif Floor Cleaner, Dettol Surface Cleaner, Finis Floor Cleaner and Lysol Surface Cleaner) were evaluated for antibacterial activity against three bacterial cultures (E. coli, K. pneumonia, S. aureus) through Quantitative non-porous Surface Carrier Test derived from EN 13697. Efficacy testing was performed through surface contamination techniques. Glass slides surfaces were artificially contaminated followed by recovery of the bacteria through vortex method both before and after the application of product for 5 minutes. Each of the experiment was repeated thrice and microbicidal effect (ME) values after the application of each product were calculated. Residual antimicrobial activity of the surface cleaning products was measured by applying the working solution of disinfectant on contaminated glass surfaces. Exposure time was given to the test surfaces, after each set exposure time the surfaces were treated to recover the microorganisms. Viable count from the eluant was calculated by serial dilution spread plate method. Each of the experiment was repeated thrice to find out the residual antimicrobial effect of the disinfectant products. ME values of the Finis Floor Cleaner ranged from 4.03 to 5.14, which was the maximum value among all surface cleaning products used against E. coli. Highest ME value against S. aureus and K. pneumonia was shown by Astonish Germ Clear. The ME values ranged from 4.99 to 5.10 against S. aureus and 5.34 to 6.99 against K. pneumoniae. Finis Floor Cleaner was proved to be of maximum efficiency against E. coli where as Astonish Germ Clear was most effective against Staph. aureus and K. pneumonia. The mean log10 CFU values recovered from disinfectant treated Summary 49 surfaces when they were exposed to the environment for different time periods of five minutes, six hours and 24 hours are 5.62 to 7.94, 4.17 to 6.35 and 7.16 to 10.25 respectively. The results indicated that the microbial count was reduced significantly at interaction time of five minutes, then at six hours the count was further reduced by Astonish Germ Clear, Cif Floor Cleaner and Dettol Surface Cleaner i.e. these surface cleaners were able to maintain their antimicrobial activity upto six hours. When the exposure to environment further increased to 24 hours, the microbial count started to increase, hence none of the disinfectants has shown antimicrobial activity upto 24 hours. This indicates that significant microbial count can be achieved within the interaction time of five minutes to six hours. Loss of antimicrobial activity upto 24 hours is probably because the active ingredients of cleaning agents get degraded during long interaction time. Present study emphasizes that the surface disinfection process must be repeated at regular intervals. Regular and timely use of surface cleaning agents must be considered as a crucial measure in controlling disease transmission rates.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2294-T (Browse shelf) Available 2294-T
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Surface disinfection plays a key role in the prevention, control and reduction of many communicable infections as contaminated surfaces are the major source for transmission of microorganisms. Five types of surface cleaning products (Astonish Germ Clear, Cif Floor Cleaner, Dettol Surface Cleaner, Finis Floor Cleaner and Lysol Surface Cleaner) were evaluated for antibacterial activity against three bacterial cultures (E. coli, K. pneumonia, S. aureus) through Quantitative non-porous Surface Carrier Test derived from EN 13697.
Efficacy testing was performed through surface contamination techniques. Glass slides surfaces were artificially contaminated followed by recovery of the bacteria through vortex method both before and after the application of product for 5 minutes. Each of the experiment was repeated thrice and microbicidal effect (ME) values after the application of each product were calculated. Residual antimicrobial activity of the surface cleaning products was measured by applying the working solution of disinfectant on contaminated glass surfaces. Exposure time was given to the test surfaces, after each set exposure time the surfaces were treated to recover the microorganisms. Viable count from the eluant was calculated by serial dilution spread plate method. Each of the experiment was repeated thrice to find out the residual antimicrobial effect of the disinfectant products.
ME values of the Finis Floor Cleaner ranged from 4.03 to 5.14, which was the maximum value among all surface cleaning products used against E. coli. Highest ME value against S. aureus and K. pneumonia was shown by Astonish Germ Clear. The ME values ranged from 4.99 to 5.10 against S. aureus and 5.34 to 6.99 against K. pneumoniae. Finis Floor Cleaner was proved to be of maximum efficiency against E. coli where as Astonish Germ Clear was most effective against Staph. aureus and K. pneumonia. The mean log10 CFU values recovered from disinfectant treated
Summary
49
surfaces when they were exposed to the environment for different time periods of five minutes, six hours and 24 hours are 5.62 to 7.94, 4.17 to 6.35 and 7.16 to 10.25 respectively. The results indicated that the microbial count was reduced significantly at interaction time of five minutes, then at six hours the count was further reduced by Astonish Germ Clear, Cif Floor Cleaner and Dettol Surface Cleaner i.e. these surface cleaners were able to maintain their antimicrobial activity upto six hours. When the exposure to environment further increased to 24 hours, the microbial count started to increase, hence none of the disinfectants has shown antimicrobial activity upto 24 hours. This indicates that significant microbial count can be achieved within the interaction time of five minutes to six hours. Loss of antimicrobial activity upto 24 hours is probably because the active ingredients of cleaning agents get degraded during long interaction time.
Present study emphasizes that the surface disinfection process must be repeated at regular intervals. Regular and timely use of surface cleaning agents must be considered as a crucial measure in controlling disease transmission rates.

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