Normal view MARC view ISBD view

Isolation And Characterization Of Avian Isolates Of Lactobacilli Species And Their Antisalmonella Activity

By: Anum Shaukat (2009-VA-220) | Dr. Muhammad Nawaz.
Contributor(s): Dr. Jawad Nazir | Prof. Dr. Mansur-Ud-Din Ahmad.
Material type: materialTypeLabelBookPublisher: 2015Description: 78p.Subject(s): Department of MicrobiologyDDC classification: 2313-T Dissertation note: Poultry industry is second largest industry of Pakistan. Poultry industry is cheaper source of protein and provides jobs for more than 1.5 million people. It is facing several problems due to microbial diseases. Salmonella is one of the leading causes of diseases in poultry. These are being treated with antibiotics but misuse and overuse of antibiotics result in antibiotic resistant strains of microorganisms. We need some alternatives for treatments. Lactobacilli are one of the alternatives to antibiotics used as probiotics. It has been found that the Lactobacilli of poultry origin have antimicrobial activity against Salmonella. Lactobacilli was isolated from the droppings, cloaca and caecum of rural poultry birds using deMan Rogosa Sharpe (MRS) medium. The isolates were screened for anti-Salmonella activity against S. enterica along with their properties to resist low pH and bile acids, antibiotic sensitivity, auto-aggregation and co-aggregation. The isolates showed anti-salmonella activity were identified using microscopic characters and biochemical profile. The isolates were confirmed by PCR using species specific primers and sequencing 16S rRNA gene. The data was analysed using one-way ANOVA at significance level P value <0.05 by using the statistical software Graph Pad Prism version 5.3. The study was conducted on a total of 60 samples including caecal swabs (n=20), cloacal swabs (n=20) and dropping (n=20) of indigenous poultry. From these samples, five isolates were Summary 57 selected based upon the tests performed. Isolates namely CLB-41, CLB-45, PDL-13, PDL-26 and PDL-33 showed best results. Further characterization was done by PCR and sequencing.
Tags from this library: No tags from this library for this title. Add tag(s)
Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2313-T (Browse shelf) Available 2313-T
Total holds: 0

Poultry industry is second largest industry of Pakistan. Poultry industry is cheaper source
of protein and provides jobs for more than 1.5 million people. It is facing several problems due
to microbial diseases. Salmonella is one of the leading causes of diseases in poultry. These are
being treated with antibiotics but misuse and overuse of antibiotics result in antibiotic resistant
strains of microorganisms. We need some alternatives for treatments. Lactobacilli are one of the
alternatives to antibiotics used as probiotics.
It has been found that the Lactobacilli of poultry origin have antimicrobial activity
against Salmonella.
Lactobacilli was isolated from the droppings, cloaca and caecum of rural poultry birds
using deMan Rogosa Sharpe (MRS) medium. The isolates were screened for anti-Salmonella
activity against S. enterica along with their properties to resist low pH and bile acids, antibiotic
sensitivity, auto-aggregation and co-aggregation. The isolates showed anti-salmonella activity
were identified using microscopic characters and biochemical profile. The isolates were
confirmed by PCR using species specific primers and sequencing 16S rRNA gene.
The data was analysed using one-way ANOVA at significance level P value <0.05 by
using the statistical software Graph Pad Prism version 5.3.
The study was conducted on a total of 60 samples including caecal swabs (n=20), cloacal
swabs (n=20) and dropping (n=20) of indigenous poultry. From these samples, five isolates were
Summary
57
selected based upon the tests performed. Isolates namely CLB-41, CLB-45, PDL-13, PDL-26
and PDL-33 showed best results. Further characterization was done by PCR and sequencing.

There are no comments for this item.

Log in to your account to post a comment.


Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:rehana.kousar@uvas.edu.pk Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.