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Polymorphism Of The Slc11a1 Gene Associated With Resistance To Bovine Tuberculosis.

By: Qamar Raza Qadri (2009-VA-569) | Dr. Asif Nadeem.
Contributor(s): Dr. Tahir Yaqoob | Dr. Abu Saeed Hashmi.
Material type: materialTypeLabelBookPublisher: 2015Description: 41p.Subject(s): Department of Molecular Biology and BiotechnologyDDC classification: 2332-T Dissertation note: Bovine Tuberculosis (bTB), caused by Mycobacterium Tuberculosis is a health threat to livestock. Information on genetic resistance or susceptibility because of polymorphisms of candidate genes could be used in making selection decisions. Solute carrier family 11 (protoncoupled divalent metal ion transporters), member 1 gene (SLC11A1), is a known candidate gene which is associated with natural resistance to infection by Mycobaterium spp in buffalo. Polymorphism in this gene can be studied for breeding disease resistance animals. Blood samples were collected from Nili Ravi buffalo breed of Pakistan. DNA was extracted by organic method. Primers were designed using Primer3 software. Amplification of gene was done by Polymerase Chain Reaction. PCR products were sequenced bi-directionally on ABI 3130XL Genetic analyzer. Sequence alignment was performed for polymorphism identification. The analysis of identified polymorphism has been done by CHROMAS software. Sequences were aligned by BLAST tool of NCBI. The results of analysis showed that no polymorphisms were identified in exonic region of gene. This might be due to less sample size. Genetics play important role in fighting against pathogens. Identifying the genes involved can lead to marker-assisted selection strategies.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2332-T (Browse shelf) Available 2332-T
Total holds: 0

Bovine Tuberculosis (bTB), caused by Mycobacterium Tuberculosis is a health threat to
livestock. Information on genetic resistance or susceptibility because of polymorphisms of
candidate genes could be used in making selection decisions. Solute carrier family 11 (protoncoupled
divalent metal ion transporters), member 1 gene (SLC11A1), is a known candidate gene
which is associated with natural resistance to infection by Mycobaterium spp in buffalo.
Polymorphism in this gene can be studied for breeding disease resistance animals. Blood samples
were collected from Nili Ravi buffalo breed of Pakistan. DNA was extracted by organic method.
Primers were designed using Primer3 software. Amplification of gene was done by Polymerase
Chain Reaction. PCR products were sequenced bi-directionally on ABI 3130XL Genetic
analyzer. Sequence alignment was performed for polymorphism identification. The analysis of
identified polymorphism has been done by CHROMAS software. Sequences were aligned by
BLAST tool of NCBI. The results of analysis showed that no polymorphisms were identified in
exonic region of gene. This might be due to less sample size. Genetics play important role in
fighting against pathogens. Identifying the genes involved can lead to marker-assisted selection
strategies.

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