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Sero-Prevalence Of Brucellosis In Sheep In Hazara Region

By: Syed Touseef Hussain Shah (2013-VA-601) | Dr. Jawaria Ali Khan.
Contributor(s): Dr. Muhammad Ayaz | Dr. Waseem Yaqoob.
Material type: materialTypeLabelBookPublisher: 2015Description: 76p.Subject(s): Department of Clinical Medicine | CMSDDC classification: 2394-T Dissertation note: The present study was conducted in two Districtsof Hazara Regioni.e. Abbottabad and Battagram District, with the collaboration of National Zoonosis Surveillance and Research Unit, Animal Sciences Institute (ASI), National Agriculture Research Centre (NARC), Islamabad, Pakistan. A total of 300 venous blood samples of 5 ml each were collect¬ed from sheep of different areas of two Districts i.e. Abbottabad and Battagram, with 150 samples from each District. Then serums were dispatched to the National Agriculture Research Centre (NARC), for the diagnosis of Brucella melitensis by Rose Bengal Plate Test (RBPT) and Indirect Enzyme-linked immunosorbent assay (I-ELISA). The prevalence of the Brucella in sheep was determined as described by (Thrusfield 2013. The prevalence of brucellosis in Hazara Region by RBPT and I-ELISA was recorded as 4.67 % and 4.33 % respectively. From 150 samples of each district, 14 (9.33 %) and 13 (8.67 %) of samples were tested positive by RBPT and I-ELISA respectively in District Abbottabad whereas, no (0 %) samples were tested positive by RBPT and I-ELISA in District Battagram. Furthermore, on Tehsil wise sero-prevalence, only 2 (2.67 %) samples out of 75 were tested positive for brucellosis in sheep by both RBPT and I-ELISA in Tehsil Havelian, while in Tehsil Abbottabad 12 (16 %) and 11 (14.67 %) samples out of 75 were tested positive for brucellosis in sheep by RBPT and I-ELISA from district Abbottabad whereas, no positive samples were detected in Tehsil Battagram and Tehsil Allai by both RBPT and I-ELISA from district Battagram. Statistically there was a significant (>0.05) difference between the prevalence of brucellosis in sheep and area of Hazara Region. The demographic variables (age, sex and breed) were analyzed using Chi-square test on SPSS version 17.0 and statistically there was no significant (>0.05) difference between the prevalence of brucellosis in sheep and demographic variables i.e. age, sex and breed. The present study has shown that brucellosis is prevalent in sheep in the Hazara region. Female animals are more sensitive than their male counterparts. Sheep of breeding age are more vulnerable than the very young ones and Balkhi breed is more prone to the disease as compared to the Kaghani and Ramghani breeds. The Sensitivity and Specificity of the diagnostic test (RBPT) was determined as described by (Thrusfield 2013). The calculated sensitivity of Rose Bengal Plate Test was 100 % and its specificity was 99.6s5 %. The results of this study will be published to communicate the interesting findings and to increase the knowledge of veterinary and public health management. Therefore, we suggest the implementation of control measures, raising public knowledge on prevention methods, a complete surveillance system for the brucellosis in Hazara Region.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2394-T (Browse shelf) Available 2394-T
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The present study was conducted in two Districtsof Hazara Regioni.e. Abbottabad and Battagram District, with the collaboration of National Zoonosis Surveillance and Research Unit, Animal Sciences Institute (ASI), National Agriculture Research Centre (NARC), Islamabad, Pakistan. A total of 300 venous blood samples of 5 ml each were collect¬ed from sheep of different areas of two Districts i.e. Abbottabad and Battagram, with 150 samples from each District. Then serums were dispatched to the National Agriculture Research Centre (NARC), for the diagnosis of Brucella melitensis by Rose Bengal Plate Test (RBPT) and Indirect Enzyme-linked immunosorbent assay (I-ELISA).
The prevalence of the Brucella in sheep was determined as described by (Thrusfield 2013. The prevalence of brucellosis in Hazara Region by RBPT and I-ELISA was recorded as 4.67 % and 4.33 % respectively. From 150 samples of each district, 14 (9.33 %) and 13 (8.67 %) of samples were tested positive by RBPT and I-ELISA respectively in District Abbottabad whereas, no (0 %) samples were tested positive by RBPT and I-ELISA in District Battagram. Furthermore, on Tehsil wise sero-prevalence, only 2 (2.67 %) samples out of 75 were tested positive for brucellosis in sheep by both RBPT and I-ELISA in Tehsil Havelian, while in Tehsil Abbottabad 12 (16 %) and 11 (14.67 %) samples out of 75 were tested positive for brucellosis in sheep by RBPT and I-ELISA from district Abbottabad whereas, no positive samples were detected in Tehsil Battagram and Tehsil Allai by both RBPT and I-ELISA from district Battagram. Statistically there was a significant (>0.05) difference between the prevalence of brucellosis in sheep and area of Hazara Region.
The demographic variables (age, sex and breed) were analyzed using Chi-square test on SPSS version 17.0 and statistically there was no significant (>0.05) difference between the prevalence of brucellosis in sheep and demographic variables i.e. age, sex and breed. The present study has shown that brucellosis is prevalent in sheep in the Hazara region. Female animals are more sensitive than their male counterparts. Sheep of breeding age are more vulnerable than the very young ones and Balkhi breed is more prone to the disease as compared to the Kaghani and Ramghani breeds. The Sensitivity and Specificity of the diagnostic test (RBPT) was determined as described by (Thrusfield 2013). The calculated sensitivity of Rose Bengal Plate Test was 100 % and its specificity was 99.6s5 %.
The results of this study will be published to communicate the interesting findings and to increase the knowledge of veterinary and public health management. Therefore, we suggest the implementation of control measures, raising public knowledge on prevention methods, a complete surveillance system for the brucellosis in Hazara Region.

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