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Activity Of Plant Extract Mediated Silver Nanoparticles Against Selective Medically Important Pathogens

By: Qurat UL Ain (2010-VA-295) | Dr. Muhammad Nawaz.
Contributor(s): Dr. Imran Najeeb | Dr. Muhammad Nasir.
Material type: materialTypeLabelBookPublisher: 2016Description: 66p.Subject(s): Department of MicrobiologyDDC classification: 2507-T Dissertation note: Due to increased use of antibiotics different common pathogenic microbes have developed antibiotic resistance. The problem of antibiotic resistance is growing day by day. Plants are being used as a valuable source of natural products to maintain the health. So use of plant extracts is an interesting and valuable way to control this problem. Metal particles have antibacterial activity. Extract mediated synthesis of metal nanoparticles is a very impressive way to control the microbial diseases. To synthesize metallic nanoparticles using extracts is a superior method than any other method. Silver has distinctive properties as antibacterial agent. Nanoparticles can be synthesized by various methods but green synthesis is environment friendly and much more important than other methods. Melia azedarach have many active components due to which its every part has some antimicrobial characteristics. Its extract mediated AgNPs are very important in this regard. Similarly Albizia procera extracts has potential to kill pathogenic microbes. That’s why these two plants were selected to synthesize nanoparticles. Fresh leaves were collected extracts were prepared. Silver nitrate solution was prepared and then this solution was allowed to react with each extract separately. After the formation, nanoparticles were separated by centrifugation. Then their characterization was done by UV visible spectroscopy and FTIR. After characterization well diffusion assay was performed to check antimicrobial activity of these nanoparticles against selected medically important pathogens. It was found that the antimicrobial activity of extract mediated nanoparticles was better than the antimicrobial activity of plant extract alone. Summary 51 In the present work it is explored that nanoparticles formed from extract of Albizia procera has a greater antimicrobial activity against important medical pathogens. In short it was proven that nanoparticles can control the pathogens in a better way as compared to the extract alone. And this result that nanoparticles mediated particles extract of Albizia procera has suggested many other new facts. Albizia procera’s properties are needed to be explored more.
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Due to increased use of antibiotics different common pathogenic microbes have developed
antibiotic resistance. The problem of antibiotic resistance is growing day by day. Plants are
being used as a valuable source of natural products to maintain the health. So use of plant
extracts is an interesting and valuable way to control this problem. Metal particles have
antibacterial activity. Extract mediated synthesis of metal nanoparticles is a very impressive
way to control the microbial diseases. To synthesize metallic nanoparticles using extracts is a
superior method than any other method. Silver has distinctive properties as antibacterial
agent. Nanoparticles can be synthesized by various methods but green synthesis is
environment friendly and much more important than other methods.
Melia azedarach have many active components due to which its every part has some
antimicrobial characteristics. Its extract mediated AgNPs are very important in this regard.
Similarly Albizia procera extracts has potential to kill pathogenic microbes. That’s why these
two plants were selected to synthesize nanoparticles. Fresh leaves were collected extracts
were prepared. Silver nitrate solution was prepared and then this solution was allowed to
react with each extract separately.
After the formation, nanoparticles were separated by centrifugation. Then their
characterization was done by UV visible spectroscopy and FTIR. After characterization well
diffusion assay was performed to check antimicrobial activity of these nanoparticles against
selected medically important pathogens. It was found that the antimicrobial activity of extract
mediated nanoparticles was better than the antimicrobial activity of plant extract alone.
Summary
51
In the present work it is explored that nanoparticles formed from extract of Albizia
procera has a greater antimicrobial activity against important medical pathogens. In short it
was proven that nanoparticles can control the pathogens in a better way as compared to the
extract alone. And this result that nanoparticles mediated particles extract of Albizia procera
has suggested many other new facts. Albizia procera’s properties are needed to be explored
more.

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