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Effect Of Synchronization Protocols (Pg And Ovsynch) On Estrus Response, Estrus Intensity, Ovulation Time And Conception Rate In Cholistani Cows

By: Muhammad Awais Ajmal (2009-VA-370) | Dr. Aijaz Ali Channa.
Contributor(s): Prof. Dr. Nasim Ahmad | Dr. Jawad Nazir.
Material type: materialTypeLabelBookPublisher: 2016Description: 40p.Subject(s): Department of TheriogenologyDDC classification: 2523-T Dissertation note: Cholistani cattle breed, like other milch breeds of our country (Sahiwal, Red Sindhi cattle), is one of the indigenous breeds of Pakistan having superior dairy characteristics. Being heat tolerant breed present in tropical and subtropical areas of Pakistan it has average milk production 1235 litters with 4.8% fat in it. To minimize the cross breeding of Cholistani cows, some efforts are being done for its conservation, through management and genetic improvement. Moreover genetic improvement, through artificial insemination (A.I) is in process. In recent times another important tool Fixed time artificial insemination (FTAI) has emerged to improve fertility which usually is performed in association with a variety of estrus and ovulation synchronization regimens. Synchronization of estrus is actually to bring large group of females in estrus at a desired fixed time by manipulation of estrus cycle. Two synchronization protocols were compared. In PG group, each animal was treated with luteolytic dose of PGF2α (d-Cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m) on random stage of the estrous cycle and repeated after 11 days TAI was done at 72 and 84 h after 2nd PG. In OVS group each cow received an intramuscular injection of gonadotropin releasing hormone (GnRH; 50 mcg of a GnRH analogue, Dalmarelin TM Fatro®, Italy; 2 ml; i.m) on random stage of estrus cycle (day 0). On day 7 these cows were treated with PGF2α (d-cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m), followed by second injection of GnRH on day 9 and TAI was done at 12 and 24h after 2nd GnRH. Estrus response and estrus intensity were higher in OVS group as compared to PG. Timing of ovulation was shorter in OVS group as compared to. Conception rate are also high in OVS group. Therefore it is concluded that Ovsynch protocol is helpful to improve ovulation and conception rate in Cholistani cows.
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Cholistani cattle breed, like other milch breeds of our country (Sahiwal, Red Sindhi cattle), is one of the indigenous breeds of Pakistan having superior dairy characteristics. Being heat tolerant breed present in tropical and subtropical areas of Pakistan it has average milk production 1235 litters with 4.8% fat in it. To minimize the cross breeding of Cholistani cows, some efforts are being done for its conservation, through management and genetic improvement. Moreover genetic improvement, through artificial insemination (A.I) is in process. In recent times another important tool Fixed time artificial insemination (FTAI) has emerged to improve fertility which usually is performed in association with a variety of estrus and ovulation synchronization regimens. Synchronization of estrus is actually to bring large group of females in estrus at a desired fixed time by manipulation of estrus cycle. Two synchronization protocols were compared. In PG group, each animal was treated with luteolytic dose of PGF2α (d-Cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m) on random stage of the estrous cycle and repeated after 11 days TAI was done at 72 and 84 h after 2nd PG. In OVS group each cow received an intramuscular injection of gonadotropin releasing hormone (GnRH; 50 mcg of a GnRH analogue, Dalmarelin TM Fatro®, Italy; 2 ml; i.m) on random stage of estrus cycle (day 0). On day 7 these cows were treated with PGF2α (d-cloprostenol 0.150 mg; Dalmazine, Fatro®, Ozzano Emilia Italy; 2 ml; i.m), followed by second injection of GnRH on day 9 and TAI was done at 12 and 24h after 2nd GnRH. Estrus response and estrus intensity were higher in OVS group as compared to PG. Timing of ovulation was shorter in OVS group as compared to. Conception rate are also high in OVS group. Therefore it is concluded that Ovsynch protocol is helpful to improve ovulation and conception rate in Cholistani cows.

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