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Studies On Biological Control Of Salmonellosis In Poultry

By: Kiran Imtiaz (2010-VA-296) | Dr. Ali Ahmad Sheikh.
Contributor(s): Prof. Dr. Masood Rabbani | Dr. Wasim Shehzad.
Material type: materialTypeLabelBookPublisher: 2016Description: 66p.Subject(s): Department of MicrobiologyDDC classification: 2511-T Dissertation note: Contaminated poultry food products are the main cause of Salmonellosis all over the world. Salmonella may enter in different poultry farms through vertical or horizontal transmission. Previously, Salmonella infection and number were controlled in poultry and their products by using different methods such as usage of hazard analysis critical control point (HACCP), antibiotics, symbiotics, etc. But not any method gives the better result that shows the reduction of Salmonella species in poultry that cause infection. Therefore, the attention was diverted to find the alternative therapies such as the usage of bacteriophage, Bacteriophage specific to Salmonella used in reduction of Salmonella number as a biocontrol agent in poultry origin. It proves effective for Salmonella reduction. In the current study samples for isolation of Salmonella and bacteriophages were collected. Salmonella was isolated from liver, caeca and lungs of infected chickens collected from different infected poultry farms. After processing of sample according to the literature method, confirmation of salmonella from samples were done by inoculating them separately on S.S agar by streaking method. Further confirmation of Salmonella was done on the basis of biochemical testing. Bacteriophage was isolated from sewage water near to the poultry farms. For their isolation, sewage sample was centrifuged and then supernatant was collected in separate tube. Filtration was done of Supernatant, and then Filtrate was used as a bacteriophage source. Bacteriophages in filtrate were confirmed by spot test method. After confirmation of both, in vivo study was performed to evaluate the effect of bacteriophage on Salmonella when inoculated in combination or separately in chickens groups. 51 Summary Statistical analysis: The data will be transferred on spreadsheet using MS Excel 2010. The data will be analyzed through one way ANOVA test using Statistical Package for Social Sciences (SPSS) version 18.0. The present study was helpful to determine the effect of bacteriophage in reduction of Salmonella in commercial broilers sector. Antibiotic resistant Salmonella infection is difficult to control using conventional ways of antibiotic therapy and is responsible for huge economic losses in poultry. Chicken groups that were used for invivo study showed that bacteriophage was proved very effective in reduction of Salmonella either gives it in combination with Salmonella or separately to poultry. It is predicted that bacteriophage therapy is better than the conventional ways for reduction of Salmonella infection in poultry sector. It is essential that the research should be continue to study the effect of bacteriophage in reduction of specie specific Salmonella, and to determine the effect of different physicochemical factor on their activity.
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Contaminated poultry food products are the main cause of Salmonellosis all over the
world. Salmonella may enter in different poultry farms through vertical or horizontal
transmission. Previously, Salmonella infection and number were controlled in poultry and
their products by using different methods such as usage of hazard analysis critical control
point (HACCP), antibiotics, symbiotics, etc. But not any method gives the better result that
shows the reduction of Salmonella species in poultry that cause infection. Therefore, the
attention was diverted to find the alternative therapies such as the usage of bacteriophage,
Bacteriophage specific to Salmonella used in reduction of Salmonella number as a biocontrol
agent in poultry origin. It proves effective for Salmonella reduction.
In the current study samples for isolation of Salmonella and bacteriophages were
collected. Salmonella was isolated from liver, caeca and lungs of infected chickens collected
from different infected poultry farms. After processing of sample according to the literature
method, confirmation of salmonella from samples were done by inoculating them separately
on S.S agar by streaking method. Further confirmation of Salmonella was done on the basis
of biochemical testing.
Bacteriophage was isolated from sewage water near to the poultry farms. For their
isolation, sewage sample was centrifuged and then supernatant was collected in separate tube.
Filtration was done of Supernatant, and then Filtrate was used as a bacteriophage source.
Bacteriophages in filtrate were confirmed by spot test method. After confirmation of both, in
vivo study was performed to evaluate the effect of bacteriophage on Salmonella when
inoculated in combination or separately in chickens groups.
51
Summary
Statistical analysis:
The data will be transferred on spreadsheet using MS Excel 2010. The data will be
analyzed through one way ANOVA test using Statistical Package for Social Sciences (SPSS)
version 18.0.
The present study was helpful to determine the effect of bacteriophage in reduction of
Salmonella in commercial broilers sector. Antibiotic resistant Salmonella infection is difficult
to control using conventional ways of antibiotic therapy and is responsible for huge economic
losses in poultry. Chicken groups that were used for invivo study showed that bacteriophage
was proved very effective in reduction of Salmonella either gives it in combination with
Salmonella or separately to poultry. It is predicted that bacteriophage therapy is better than
the conventional ways for reduction of Salmonella infection in poultry sector. It is essential
that the research should be continue to study the effect of bacteriophage in reduction of
specie specific Salmonella, and to determine the effect of different physicochemical factor on
their activity.

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