Antibiotic resistance is an international public health problem as a result of an excessive and indiscriminate antibiotics usage, which has resulted in emergence of MDR microorganisms. This study is designed for the evaluation of antibacterial activity of different dilutions of Diclofenac sodium alone and in combination with Ciprofloxacin by using well diffusion method against bacterial pathogens to improve the quality of life of patients and minimize the chances of infections.
Different dilutions of Diclofenac sodium alone and in combination with Ciprofloxacin were checked for antibacterial activity againstEscherichia coli, Staphylococcus aureus, Klebseilla pneumonia and Salmonella enterica. Three isolates of each bacterium were obtained from Microbiology department, University of Veterinary and Animal sciences, Lahore. The pathogens were tested for their sensitivity to ciprofloxacin, amoxicillin and azithromycin. The sensitivity was checked by disc diffusion method. Their zones of inhibition was measured in mm and compared with the CLSI standards. All the available bacteria were sensitive to ciprofloxacin.
Well diffusion method was used to measure the antibacterial activity of ciprofloxacin. For this purpose, 100 µg of ciprofloxacin was used and three concentrations of 100µg, 200µg and 300µg of diclofenac sodium were used. Diclofenac sodium when used with the ciprofloxacin in higher concentrations, it caused the increase in the zone of inhibition significantly among all the bacteria. Maximum antibacterial activity of drugs combination was seen with E.coliamong all the bacteria.Synergism between diclofenac sodium and ciprofloxacin was found to be statistically significant (P ≤ 0.01) when compared with the individual effects of the ciprofloxacin
Collected data was analyzed by using statistic package for social sciences (SPSS, windows version, Chicago, IL, USA). Analysis of Variance (ANOVA) and descriptive statistics was appliedusing graphpad prism 5.03.
This study indicates that Diclofenac sodium in combination with Ciprofloxacin against Escherichia coli,Staphylococcusaureus, Klebseilla pneumonia and Salmonella entericamay be effective and used as a synergistic combination to achieve more effective outcomes for disease management. This can be experimented further to evaluate these effects and prove to be major leap towards the increasing antibacterial resistance globally.