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Polymorphism Analysis Within Tata-Box Of Bovine Lactoferrin Gene And Its Association With Mastitis In Sahiwal Cows

By: Kashmala Haroon (2014-VA-04) | Dr. Sehrish Firyal.
Contributor(s): Dr. Immad Rashid | Dr. Muhammad Tayyab.
Material type: materialTypeLabelBookPublisher: 2016Description: 52p.Subject(s): Molecular Biology and BiotechnologyDDC classification: 2584-T Dissertation note: Mastitis is one of the most important diseases in dairy cows throughout the world, and is responsible for significant economic losses to the dairy industry especially in Pakistan. Several factors are responsible for this disease and about 20% bovines are suffering with this disease. Mastitis susceptibility and resistance is influenced by genetic variation of animals. Variations to polymorphisms in LF gene assume critical part of the immune response to mastitis. Polymorphism within LF gene may influence immune response to the mastitis in bovines. Recent study shows that promoter region of LF gene is highly polymorphic among bovines. Present study was planned to identify polymorphism analysis within TATA-box of bovine LF gene and its association with mastitis. Multiple blood samples were collected from Sahiwal cows having clinical and sub-clinical mastitis. 10 samples were collected as a control. DNA extraction was done by organic extraction method and then quantification was done by Nanodrop. Amplification and sequencing was performed to get desire sequence of the gene. Comparative study of obtained sequence results were analyzed by using NCBI blast. Bioinformatics analysis was done with the help CLUSTAL W and BioEdit softwares. Two novels and one reported SNPs were discovered within TATA-box of LF gene that might be having strong genetic association with mastitis in Sahiwal cows. This gene is strong candidate gene to differentiate between mastitis susceptible and resistant Sahiwal cows.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2584-T (Browse shelf) Available 2584-T
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Mastitis is one of the most important diseases in dairy cows throughout the world, and is responsible for significant economic losses to the dairy industry especially in Pakistan. Several factors are responsible for this disease and about 20% bovines are suffering with this disease. Mastitis susceptibility and resistance is influenced by genetic variation of animals. Variations to polymorphisms in LF gene assume critical part of the immune response to mastitis. Polymorphism within LF gene may influence immune response to the mastitis in bovines. Recent study shows that promoter region of LF gene is highly polymorphic among bovines.
Present study was planned to identify polymorphism analysis within TATA-box of bovine LF gene and its association with mastitis. Multiple blood samples were collected from Sahiwal cows having clinical and sub-clinical mastitis. 10 samples were collected as a control. DNA extraction was done by organic extraction method and then quantification was done by Nanodrop. Amplification and sequencing was performed to get desire sequence of the gene. Comparative study of obtained sequence results were analyzed by using NCBI blast. Bioinformatics analysis was done with the help CLUSTAL W and BioEdit softwares.
Two novels and one reported SNPs were discovered within TATA-box of LF gene that might be having strong genetic association with mastitis in Sahiwal cows. This gene is strong candidate gene to differentiate between mastitis susceptible and resistant Sahiwal cows.

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