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Polymorphism Analysis Of Exon 2,5 And 10 Of Bovine Lactoferrin Gene And Its Association Within Mastitis In Sahiwal Cows

By: Sidra Mukhtar (2014-VA-223) | Dr. Sehrish Firyal.
Contributor(s): Dr. Sultan Ali | Dr. Muhammad Wasim | Dr. Muhammad Avais.
Material type: materialTypeLabelBookPublisher: 2016Description: 76p.Subject(s): Molecular Biology & BiotechnologyDDC classification: 2582-T Dissertation note: A few factors militate against understanding the milk production capability of bovines. Mastitis is the most costly and the prevalent production-limiting disease of dairy animals in Pakistan and somewhere else on the world. Susceptibility and resistance to mastitis is a complex characteristic and impacted by hereditary variation of animals. Among these variations, the polymorphisms in LF assumes critical part in the immune responses to mastitis. Susceptibility and resistant to mastitis is a complex trait and influenced by genetic variation of the immunity genes of the animals. Among these variations, polymorphism in Lactoferrin gene (LF) play important role in immune responses to mastitis. Polymorphism in exons 2, 5 and 10 of Lactoferrin gene are associated with mastitis susceptibility and resistance. It is a potential candidate gene for screening of the mastitis susceptible and resistant dairy cows. The present study was designed for the identification of polymorphism in LF gene associated with mastitis. Blood samples from 20 Sahiwal cows having clinical and subclinical mastitis were sampled. Blood samples of 10 normal Sahiwal cows were also collected. DNA was extracted. Specific primers for amplification of LF gene were designed by using Primer 3 software. LF gene was amplified and sequenced to get the full length sequence of the gene. Comparative analysis of resulted sequences was done with the help of NCBI BLAST. Multiple sequence alignment was done by using CLUSTAL W and BioEdit softwares. Protein analysis was done with ExPasy translate tool and the development of 3D structure were using PYMOL software.
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Item type Current location Collection Call number Status Date due Barcode Item holds
Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2582-T (Browse shelf) Available 2582-T
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A few factors militate against understanding the milk production capability of bovines. Mastitis is the most costly and the prevalent production-limiting disease of dairy animals in Pakistan and somewhere else on the world. Susceptibility and resistance to mastitis is a complex characteristic and impacted by hereditary variation of animals. Among these variations, the polymorphisms in LF assumes critical part in the immune responses to mastitis.
Susceptibility and resistant to mastitis is a complex trait and influenced by genetic variation of the immunity genes of the animals. Among these variations, polymorphism in Lactoferrin gene (LF) play important role in immune responses to mastitis. Polymorphism in exons 2, 5 and 10 of Lactoferrin gene are associated with mastitis susceptibility and resistance. It is a potential candidate gene for screening of the mastitis susceptible and resistant dairy cows.
The present study was designed for the identification of polymorphism in LF gene associated with mastitis. Blood samples from 20 Sahiwal cows having clinical and subclinical mastitis were sampled. Blood samples of 10 normal Sahiwal cows were also collected. DNA was extracted. Specific primers for amplification of LF gene were designed by using Primer 3 software.
LF gene was amplified and sequenced to get the full length sequence of the gene. Comparative analysis of resulted sequences was done with the help of NCBI BLAST. Multiple sequence alignment was done by using CLUSTAL W and BioEdit softwares. Protein analysis was done with ExPasy translate tool and the development of 3D structure were using PYMOL software.

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