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Effect Of Methionine And Mannan Oligosaccharides Supplementation On Growth Performance, Carcass Characteristics, Immunity And Selected Blood Metabolites In Broiler Chicken Under Conditions Of High Ambient Temperature And Humidity

By: Asif Mahmood (2009-VA-379) | Dr. Muhammad Afzal Rashid.
Contributor(s): Prof. Dr. Talat Naseer Pasha | Prof. Dr. Athar Mahmud.
Material type: materialTypeLabelBookPublisher: 2016Description: 56p.Subject(s): Animal NutritionDDC classification: 2606-T Dissertation note: Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine SUMMARY 42 (1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet. SUMMARY 43 SUGGESTION AND RECOMMENDATION Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity.
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Poultry sector is one of the most organized and vibrant segment of agriculture sector in Pakistan and is claimed to be among the largest industries of Pakistan with over more than 200 billion rupees investment and offering employment directly or indirectly to 1.5 million people. Broilers exposed to high environmental temperature and humidity exhibit several physiological and performance related setbacks. Heat stress cause high mortality, poor FCR, reduced feed consumption, body weight gain, and visceral development. Heat stress has been linked to a decrease in intestinal immune activity and to impaired intestinal morphology following pathogenic bacteria invasion of the body through the intestinal epithelium. Nutritional manipulations with its low cost are preferred to alleviate the negative effects of heat stress on performance. Nutritional strategies such as the addition of prebiotics, some amino acids e.g. methionine are reported to be beneficial in alleviating heat stress. As a nutritional intervention, synthetic methionine added to the diet has been explored as method of ameliorating the effects of ROS associated with high environmental temperature. Keeping above in view, the present study has been planned to evaluate the effect of supplementing methionine and MOS on the growth performance, carcass characteristics and immune status against ND under field conditions. In this experiment a total of n=200 day old chicks were procured from a commercial hatchery and randomly assigned to dietary treatments with n=40 chicks per treatment. Each treatment was further divided into four replicates with n=10 birds/replicate. Dietary treatments were : 1) negative control (NC) without any supplementation, 2) positive control (PC) containing antibiotic, 3) MOS supplemented (MOS) containing 800 and 400 g/ton of MOS in the starter and grower, respectively, 4) High Methionine supplemented treatment (HM) containing Methionine 1% of the diet in both starter and grower feeds, 5) Methionine and MOS supplemented (HM+MOS) treatment containing high methionine
SUMMARY
42
(1% in starter and grower) along with 800 and 400 g/ton of MOS in the starter and grower, respectively. The temperature and humidity were recorded daily at 8:00, 14:00 and 18:00 h and means were calculated to determine the daily changes in temperature humidity index. Feed intake, body weight and FCR were recorded at the end of each week. At the end of experiment n=3 birds per replicate were selected, weighed and slaughtered. At the time of slaughtering blood samples were collected in evacuated tubes. Serum was harvested after centrifugation, dived into aliquots and stored at –20 oC until further analysis. Serum samples were used to determine antibody titer against ND. At the time of slaughtering organ weighed and jejunal sample were collected to determine the gut morphology. The data collected were analyzed using analysis of variance (ANOVA) technique in a Completely Randomized Design (CRD). Results were declared statistically significant at P < 0.05 and compared using Tukey, s multiple comparison test. The statistical analysis were carried out using SAS 9.1 (SAS Institute, 2006). Live BW was higher (P<0.05) in HM+MOS compared to NC and HM. Feed intake was higher (P<0.05) in HM+MOS and PC than those in NC. However, FCR was lowest in HM+MOS compared to NC and PC birds. Relative organ weights were similar (P>0.05) among treatments; however, bursa of fabricius was heavier in MOS compared to the NC and HM+MOS. Villus height and villus to crypt ratio was highest (P<0.05) in HM, than the NC treatment birds CONCLUSIONS In conclusion, supplementation of methionine at 1% of diet and MOS improved the weight gain, feed intake and feed conversion ratio in birds reared under conditions of high temperature and humidity. Gut morphology of the bird also improved by supplementation of Methionine and MOS in the broiler diet.
SUMMARY
43
SUGGESTION AND RECOMMENDATION
Further research is needed to re-determine the recommended levels of methionine in the diet when bird are reared under the conditions of high temperature and humidity.

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