Brucellosis is one of the most important zoonotic diseases of large and small ruminants with worldwide distribution. Brucellosis is declared as serious problem in 86 countries as it is widely distributed throughout the developing world (WHO, 1996). It mainly affects sexually mature animals and causes significant reproductive losses (Wadood et al. 2009). In Azad Jammu & Kashmir (AJK), research work on seroprevalence of brucellosis in small ruminants is very limited. In Bhimber Azad Jammu and Kashmir, 13.33% prevalence of brucellosis in goats has been reported (Din et al. 2013). In another study in Kashmir valley, overall 6.5% small ruminants were found positive by RBPT (STAT 2013). The data was based only on screening test. No confirmatory test like ELISA or PCR was performed to know the true picture of disease problem among RBPT positive ones. To find out seroprevalence of brucellosis in small ruminants, 400 animals ( n= 173 sheep, n=227 goat) were randomly selected and were screened for brucellosis in District Bagh Azad Jammu and Kashmir. A questionnaire was filled for each animal which carried entries including breed, specie, sex, lactation status, abortion history, housing, feeding, age and management etc. Sera samples from these animals were collected and examined by RBPT. Sera samples declared positive by RBPT were subjected to further analysis through ELISA. All lab analyses were done at University diagnostic Lab UVAS, Lahore. The data originated from this study was tested by using Chi square by using “SPSS version 20” and probability level <0.05 was considered significantly different. The results showed overall prevalence 30.75% through RBPT while 2.25 % by ELISA in sheep and goats of District Bagh Azad Jammu and Kashmir. The results showed that the prevalence through RBPT was 14.7 % in
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sheep and 16 % in goats while through ELISA it was 1 % and 1.25 % in sheep and goats respectively.
The results revealed that overall prevalence was higher in goats than in sheep. Moreover the prevalence was higher in female then in male. The prevalence of brucellosis was higher in age group (1.6 Y to 3 Y) in both sheep and goats. The results showed that prevalence was higher in non-pregnant as compare to pregnant animals while prevalence was higher in dry as compare to lactating sheep and goats. Area wise results revealed that prevalence was higher in Bagh followed by Harighel, Arja and Mongbajri.