TY - BOOK AU - Misbah Sajjad (2010-VA-299) AU - Dr. Aamir Ghafoor AU - Dr.Muhammad Hassan Mushtaq ED - TI - Study On The Diversity And Antibiotic Resistance Profile Of Bacteria Isolated From Respiratory Tract Infected Dogs And Human U1 - 2696-T PY - 2016/// KW - Microbiology N1 - In developing countries, respiratory tract infections are the major reason of the prevalence of disease and death in the patients who are seriously sick. Respiratory problems in human and dogs are common in Pakistan. Broad spectrum antibiotics are being used to treat the infections but duration of illness is increasing day by day. Present study was therefore designed to explore the diversity of bacteria causing respiratory tract infections in dogs and human and antibiotic resistance pattern of bacterial isolates Total 60 Samples (sputum, nasal discharge, tracheal swab etc) were collected and evaluated. Thirty (n=30) samples were taken from human and dog each. Among 30 samples of human, 10 were taken from infected males and females each. 5 samples from healthy males and females each were also included. Similarly among 30 samples of dogs, 10 were taken from infected dogs and bitches each along with 5 samples from healthy dogs and bitches each. Samples collected from each gender of human and dog were cultured on the MacConkey, Blood and Chocolate agar plates for the isolation of pathogens. Isolated were confirmation by biochemical tests followed by determination of their antibiotic susceptibility pattern by using Kirby Baur disk diffusion method. From male samples, Staph spp were isolated from all the samples (100%) followed by E.coli (70%), and Salmonella spp, Haemophilus spp each (30%). Whereas from females, Staph aureus were also isolated from 100% samples followed by Salmonella spp (60%), E.coli (40%), and Haemophilus spp (0%) Summary 68 From healthy males, isolation of Staph aureus was confirmed in (100%) samples followed by Salmonella spp (80%), E.coli (20%), and Haemophilus spp (0%). Whereas from females, Staph aureus was also confirmed in (100%) samples followed by E.coli (80%), Salmonella spp (20%) and Haemophilus spp (0%). From dog samples, Staph aureus was isolated from (100%) samples followed by E.coli (60%), Salmonella spp (40%) and Haemophilus spp (20%). Whereas from bitches, Staph aureus was also isolated from (100%) samples followed by Salmonella spp (60%), E.coli (50%), and Haemophilus spp (0%) From the samples of healthy dogs, Isolation of Staph aureus was confirmed in (100%) samples followed by E.coli (80%), Salmonella spp (20%) and Haemophilus spp (0%). Whereas from bitches, Staph aureus was also isolated from (100%) samples followed by Salmonella spp (80%), E.coli (20%), and Haemophilus spp (0%) Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected dogs showed 100% resistance to Amoxicillin, Tylosine and Lincomycin and 100% sensitivity to Doxycycline and Oxytetracycline. E.coli showed 100% resistance to Amoxicillin and Lincomycin and 80.8% sensitivity to Ciprofloxacin. Staph spp showed 100% resistance to Tylosine and Lincomycin and Haemophilus spp showed 100% resistance to Ciprofloxacin and Doxycycline Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy dogs showed 100% resistance to Tylosine and Lincomycin. E.coli showed 100% resistance to Tylosine and Lincomycin and 75.8% sensitivity to Ciprofloxacin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 75% sensitivity to Doxycycline. There was no Summary 69 positivity observed for Haemophilus spp in all samples of healthy dogs, so no sensitivity was performed Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected bitches showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. There was no positivity observed for Haemophilus spp in all samples of infected bitches, so no sensitivity was performed Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy bitches showed 100% resistance to Tylosine and Lincomycin and 90% sensitivity to Doxycycline. E.coli showed 100% resistance to Amoxicillin, Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin and 70% sensitivity to Doxycycline. There was no positivity observed for Haemophilus spp in all samples of healthy bitches, so no sensitivity was performed Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected males showed 80% resistance to Amoxicillin and Tylosine. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Lincomycin and 100% sensitivity to Doxycycline. Haemophilus spp showed 100% sensitivity to Doxycycline Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy males showed 100% resistance to Lincomycin. E.coli showed 100% resistance to Tylosine and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin. There was no Summary 70 positivity observed for Haemophilus spp in all samples of healthy males, so no sensitivity was performed Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from infected females showed 100% resistance to Amoxicillin, Tylosine and Lincomycin. E.coli showed 100% resistance to Amoxicillin and Lincomycin. Staph spp showed 100% resistance to Lincomycin. There was no positivity observed for Haemophilus spp in all samples of infected females, so no sensitivity was performed Antibiotic susceptibility pattern of Salmonella spp isolated from samples taken from healthy females showed 100% resistance to Lincomycin. E.coli showed 100% resistance to Amoxicillin and Lincomycin. Staph spp showed 100% resistance to Tylosine and Lincomycin. There was no positivity observed for Haemophilus spp in all samples of healthy females, so no sensitivity was performed Conclusion Our study showed that samples of respiratory tract collected from human and dog showed involvement of bacteria such as Salmonella spp, E. coli and Staph aureus and Haemophilus spp. Most of strains were multidrug resistant. Ciprofloxacin, Doxycycline and Oxytetracycline were effective to treat respiratory infections in human and dogs ER -