01669nam a2200205Ia 4500
20150917155010.0
150525s1997 xx 000 0 und d
eng
0541,T
Ghulam Nabi
5901
Dr. Khushi Muhammad
5902
Preparation And Evalution Of Oil Based Egg Drop Syndrome Virus Vaccine
1997
The egg drop syndrome virus (Pak-CVS-EDSV: collected from Mirobiology Setion, CVS, Lahore) was culturally characterised by inoculating in embryonated duck eggs, and by haemagglutination (HA) potential of only avian RBC and virus neutralization test. The virus was found antigenically related with the imported vaccinal strain of EDS virus. The virus grew well in the embryonated duck eggs. The HA titer of Allanto-Amniotic fluid (AAF) was more than log 211, while its E1D50 was determined to be 10-10.37 per ml.
An oil-based EDSV vaccine was prepared by mixing one part of the AAF with 4 parts of the oil-base. The oil base contained 4% emulsifier (Span-80). The vaccine thus prepared from the local isolate was antigenically comparable with the imported vaccine. The cost of the vaccine production using local strain of the EDS virus was Rs.463/bottle (1000 doses) compared to Rs.1650/bottle of the imported vaccine. The price of the one ml diagnostic antigen was calculated at Rs.20/- compared to Rs.600 per one ml of imported antigen (Market price is Rs.2200/ml of the antigen).
Department of Microbiology
4609
Dr. Muhammad Akram Munieer
5903
Dr. Shakil
5440
Faculty of Veterinary Sciences
4292
TH
2286
2286
0
0
0
0541T
0
VS
3048
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2015-05-27
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2015-05-27
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