TY - BOOK AU - Zahid Jawad AU - Dr. Khushi Muhamma AU - Dr.Javed Rashid ED - ED - Faculty of Veterinary Sciences TI - Detection And Control Of Vaccination Stress Following Vaccination With Live Virus Newcastle Disease Vaccine And Its Effect on Immune Response Commmercial Broiler Chicks U1 - 0806,T PY - 1997/// KW - Department of Pathology N1 - This study intended to ascertain the vaccination stress following live Newcastle disease vaccine by oral route and to determine the comparative efficacy of probiotic and vitamins to combat it. One hundred and forty day-old broiler chicks were divided into four groups i.e. A, B, C and D having 35 birds each. The birds were kept for 45 days alter vaccination with Bio-LaSota by oral route on 21st day. Chicks From group A was kept as control. Group B was given vaccine and no treatment. Group C was given vaccine and only brobiotic (protexin). Group D was given vaccine and only vitamins (Vitaoligosol). Seven birds of each group were slaughtered on 22nd day, 23th, 24th and 25th day and remaining 7 birds of each group were slaughtered on day 45th, for collection of blood samples. The blood samples from 7 randomly selected birds were collected on day 16, 30th and day 45th for determination of antibody t.itre. The following parameters were studied: (i) determination of hetrophil/lymphocyte ratio (Ii) estimation of antibody response against Newcastle disease vaccine (iii) estimation of serum biochemical substances (iv) Determination of adrenal gland body weight index and (v) Pathological study of adrenal glands. Live virus vaccine against Newcastle disease caused vaccination stress in broiler chickcns. The birds expressed vaccination stress for a variable period ranged from 1 to 2 post-vaccination day. But these results were more accurate if level of corticosteroids was detected. In future, a more comprehensive study is required to devise simple and reliable methods for detecting stress in poultry in field conditions and also to suggest therapeutic and prophylactic measures for relieving the birds from the state of stress. ER -