Diagnosis Of Paratuberculosis (Johne,S Disease) In Cattle And Buffaloes Through Histopathological Techniques And Polymerase Chain Reaction
Farhan Anwar Khan 5737
Dr.Zafar Iqbal Ch. 5738
Dr.Asim Aslam 5682
Prof .Dr.Khushi Muhammad 16359
Faculty of Veterinary Sciences 4292
Department of Pathology 4635
1011,T
Paratuberculosis, one of the infectious disease, is the emerging cause of poor health, low productivity and finally death due to single infectious agent among dairy and beef yielding animals (cattle and buffaloes) in the World. Mycobacterium avium subsp. paratuberculosis is the most common cause of bovine Johne's disease.
The study was conducted in Lahore to compare conventional methods and PCR for the diagnosis of paratuberculosis caused by M avium subspp. paratuberculosis in 300 cattle's and buffalo's tissue samples (150 of each specie), including terminal ileum and mesenteric lymph nodes. Conventional methods included Ziehi-Neelsen's (ZN) acid fast staining and histopathology. For M paratuberculosis insertion sequence IS 900, specific 626 bp fragment, were targeted. The sensitivity and specificity of PCR was found significant in comparison to Ziehl Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes.
2008
2008
2008
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eng