TY - BOOK AU - Farhan Anwar Khan AU - Dr.Asim Aslam AU - Prof .Dr.Khushi Muhammad ED - ED - Faculty of Veterinary Sciences TI - Diagnosis Of Paratuberculosis (Johne,S Disease) In Cattle And Buffaloes Through Histopathological Techniques And Polymerase Chain Reaction U1 - 1011,T PY - 2008/// KW - Department of Pathology N1 - Paratuberculosis, one of the infectious disease, is the emerging cause of poor health, low productivity and finally death due to single infectious agent among dairy and beef yielding animals (cattle and buffaloes) in the World. Mycobacterium avium subsp. paratuberculosis is the most common cause of bovine Johne's disease. The study was conducted in Lahore to compare conventional methods and PCR for the diagnosis of paratuberculosis caused by M avium subspp. paratuberculosis in 300 cattle's and buffalo's tissue samples (150 of each specie), including terminal ileum and mesenteric lymph nodes. Conventional methods included Ziehi-Neelsen's (ZN) acid fast staining and histopathology. For M paratuberculosis insertion sequence IS 900, specific 626 bp fragment, were targeted. The sensitivity and specificity of PCR was found significant in comparison to Ziehl Neelsen staining and histopathology for the diagnosis of paratuberculosis in cattle and buffaloes ER -