01973nam a2200193Ia 4500
20151005130747.0
150525s2011 xx 000 0 und d
eng
1239,T
Muhammad Tayyub
7468
Prof. Dr. Kamran Ashraf
5070
Antigenic Relatedness Of Caecal Eimeria Species In Broilers By Sds-Page
2011
Coccidiosis is caused by various species of Eimeria. Avian coccidiosis is divided in two types (intestinal and caecal). Eimeria tenella and E. necatrix cause caecal eimeriosis.E.tenella is the most pathogenic species and its infection is causing huge economic losses to poultry industry world wide. In the present study, 400 caecal samples suspected for coccidiosis were collected from two districts (Kasur and Sheikhupura). Ten samples from twenty farms of each district were collected and examined for coccidiosis and species identication. The prevalence of coccidiosis was higher (65.25%) in both districts. Coccidiosis was found more prevalent in district Kasur (66.5%) as compared to Sheikhupura (63%). Eimeria species were identified by conventional methods like Direct Microscopy, Sedimentation technique, Floatation Technique and Sporulation.. The prevalence percentage of E.tenella and E,necatrix was found 67.5% and 12.25% respectively in 400 suspected caecal samples. E.tenella was more prevalent in district Kasur (67.5%) as compared to district Sheikhupura (63%). E.necatrix was also more prevalent in district Kasur (13%) than district Sheikhupura (11.5%). Protein profiling of all E. tenella strains was performed to check antigenic related ness between different isolates of E.tenella. More tan ninety percent isolates were antigenically identical which showed that E.tenella isolates in both districts were antigenically related.
Department of Parasitology
4290
Dr. Aftab
6259
Dr. Nisar Ahmad
4438
TH
3004
3004
0
0
0
1239T
0
VS
3823
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2015-05-28
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2015-05-28
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